Exosome-Transmitted miR-506-3p Inhibits Colorectal Cancer Cell Malignancy via Regulating GSTP1.

Abstract

Exosome-mediated microRNA transfer has been shown to regulate cancer progression. However, the involvement of exosomal-miR-506-3p in colorectal cancer (CRC) is unknown. The goal of the research was to study into the role of exosomal-miR-506-3p in CRC. Using a qRT-PCR experiment, it was observed that CRC tissues had lower levels of miR-506-3p than non-tumor tissues. It was observed that miR-506-3p inhibited the proliferation, regulates apoptosis, and cell cycle of HT29 and SW480 cells as compared to control groups. Dual luciferase reporter assay results showed that GSTP1 was the downstream target molecule of miR-506-3p, which was consistent with the database prediction. Furthermore, FHC cells transfected with miR-506-3p could transfer miR-506-3p to SW480 cells, limiting cell growth and inducing cell death. We discovered a unique regulatory mechanism in which exosome-mediated transfer of miR-506-3p reduces proliferation and induces apoptosis in CRC through negative regulation of GSTP1, implying that exosome-mediated delivery of miR-506-3p provides fresh insight into CRC diagnostics and treatment.