Abstract
BackgroundWound healing is a complex pathophysiological process that involves a variety of cells and cytokines. In this study, we found that local injection of human amnion mesenchymal stem cells into wounds in rats could promote wound healing. Therefore, we hypothesized that the exosomes of human amnion mesenchymal stem cells contain substances that regulate the migration of epidermal cells. It has been reported that miR-135a is involved in cell migration and transformation. However, there have been no reports of its function in skin wound healing.MethodsTo test this hypothesis, we injected exosomes overexpressing miR-135a directly into the wound margin. In addition, we tested the migration of BJ cells with overexpression or knockdown of miR-135a in vitro. Additionally, Western blot analysis was used to detect the expression of fibroblast migration-associated proteins after treatment with miR-135a overexpression or knockdown.ResultsMiR-135a significantly promoted wound healing compared to the control treatment. Western blot analysis showed a significant downregulation of LATS2 after overexpression of miR-135a. In addition, knockdown of miR-135a effectively attenuated the promoting effect of exosomes on cell migration.ConclusionsOur results indicated that miR-135a promotes wound healing, which may be mediated by downregulating LATS2 levels to increase cell migration. This study provides a rationale for the therapeutic effect on wound healing of miR-135a in exosomes derived from human amnion mesenchymal stem cells.
Highlights
Skin wound healing is a complex process involving a number of highly coordinated steps, mainly divided into inflammatory response, epithelialization and wound contraction, collagen deposition, and remodeling [1,2,3]
The results showed that Human amnion mesenchymal stem cell (hAMSC) could promote wound healing
In this study, we found that local injection of human amnion mesenchymal stem cells into wounds in rats could promote wound healing
Summary
Skin wound healing is a complex process involving a number of highly coordinated steps, mainly divided into inflammatory response, epithelialization and wound contraction, collagen deposition, and remodeling [1,2,3]. Some progress has been made in the study of wound healing, Gao et al Stem Cell Research & Therapy (2020) 11:56 experimental results have confirmed that promoting keratinocyte migration can accelerate wound healing. Current research indicates that miR-135a can promote the migration abilities of breast cancer cells [12]. It is necessary to carry out systematic and in-depth research on these problems, which is of great significance for clarifying the mechanism of wound healing and exploring new targets for clinically promoting wound healing. Wound healing is a complex pathophysiological process that involves a variety of cells and cytokines. We found that local injection of human amnion mesenchymal stem cells into wounds in rats could promote wound healing. We hypothesized that the exosomes of human amnion mesenchymal stem cells contain substances that regulate the migration of epidermal cells. There have been no reports of its function in skin wound healing
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