Abstract
BackgroundExosomes mediated transfer of lncRNA 91H may play a critical role in the development of CRC. However, few studies have proved the mechanism. So we performed this study to deeply explore the biological functions of exosomal 91H in the development and progression of CRC.MethodsThe association between lncRNA 91H and exosomes was detected in vitro and vivo. Then RNA pulldown and RIP were used to detect how lncRNA 91H affect CRC IGF2 express. At last, clinic pathological significance of exosomal 91H was evaluated by Cox proportional hazards model.ResultsWe found that serum lncRNA 91H expression was closely related to cancer exosomes in vitro and vivo which may enhance tumor-cell migration and invasion in tumor development by modifying HNRNPK expression. Then the clinic pathological significance of exosomal 91H was evaluated which demonstrated that CRC patients with high lncRNA 91H expression usually showed a higher risk in tumor recurrence and metastasis than patients with low lncRNA 91H expression (P < 0.05).ConclusionAll these data suggested that exosomal lncRNA 91H enhancing CRC metastasis by modifying HNRNPK expression might be an early plasma-based biomarker for CRC recurrence or metastasis. Further large-scale studies are needed to confirm our findings.
Highlights
Exosomes mediated transfer of long noncoding RNAs (lncRNAs) 91H may play a critical role in the development of Colorectal cancer (CRC)
The association between lncRNA 91H and exosomes By performing qRT-PCR, the expression of exosomal 91H in cell lines HCT-8, HCT-116 were 16.96 and 5.30fold change higher than normal human intestinal epithelial cell line (FHC) with P < 0.05 (Fig. 2a)
The expression of lncRNA 91H in exosomes was compared with other places of HCT-116 medium which demonstrated that the expression of lncRNA 91H in exosomes was 4.72-fold change higher than other places of cell medium (Fig. 2b, P < 0.05)
Summary
Exosomes mediated transfer of lncRNA 91H may play a critical role in the development of CRC. Gao et al Cancer Cell Int (2018) 18:11 that exosomes play critical roles in causing deregulated local and systemic cellular communication in the tumor microenvironment. Recent studies have demonstrated that by being assembled into cancer exosomes, lncRNAs could be secreted from tumor cells into body fluids such as blood, urine, milk, and saliva [12, 13]. These tumor-derived exosomes in turn enhanced tumor cell invasiveness and metastasis [14]. It was proved that lncRNA HOTAIR from patients with high-grade muscle-invasive disease are enriched in exosomes than other patients in urothelial bladder cancer [16]
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