Abstract
Pseudomonas aeruginosa is the most common human opportunistic pathogen associated with nosocomial diseases. In 2017, the World Health Organization has classified P. aeruginosa as a critical agent threatening human health, and for which the development of new treatments is urgently necessary. One interesting avenue is to target virulence factors to understand P. aeruginosa pathogenicity. Thus, characterising exoproteins of P. aeruginosa is a hot research topic and proteomics is a powerful approach that provides important information to gain insights on bacterial virulence. The aim of this review is to focus on the contribution of proteomics to the studies of P. aeruginosa exoproteins, highlighting its relevance in the discovery of virulence factors, post-translational modifications on exoproteins and host-pathogen relationships.
Highlights
Pseudomonas aeruginosa is a common environmental Gram-negative bacterium, which is part of the Pseudomonadaceae
They compared the wild type (WT) and the ∆tat mutant exoproteomes using Differential Gel Electrophoresis (DIGE) followed by protein identifications by MALDI/MS
The infection microenvironment can influence the expression of virulence factors in P. aeruginosa. These examples have clearly shown that proteomic studies of bacterial supernatants have the ability to reveal proteins as potential virulence factors, which would not have been suspected without these analyses, as hypothetical proteins
Summary
Pseudomonas aeruginosa is a common environmental Gram-negative bacterium, which is part of the Pseudomonadaceae. ExoS on the bacterial surface, when the bacterium is next to host cells [12] It injects toxins directly in the and ExoT target and inactivate different substrates including GTPases and adaptor proteins, which cytoplasm of target cells and four exotoxins have been identified in aeruginosa strains: ExoS, ExoT, trigger actin cytoskeleton dismantlement and cell apoptosis [12]. P. aeruginosa in different conditions, which have contributed broadly to the understanding of bacterial physiology and virulence [26,27,28,29,30,31] These studies miss an important subset of proteins that are secreted into the extracellular milieu [32]. The last section will consider the contribution of proteomics to a better understanding of host-pathogen relationships
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