Abstract
Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. We examined the genomes and exoproteomes of two multilocus sequence type (MLST) clade 2 C. difficile strains considered hypervirulent: ICC-45 (ribotype SLO231/UK[CE]821), isolated in Brazil, and NAP1/027/ST01 (LIBA5756), isolated during a 2010 outbreak in Costa Rica. C. difficile isolates were cultured and extracellular proteins were analyzed using high-performance liquid chromatography-tandem mass spectrometry. Genomic analysis revealed that these isolates shared most of the gene composition. Only 83 and 290 NAP1/027 genes were considered singletons in ICC-45 and NAP1/027, respectively. Exoproteome analysis revealed 197 proteins, of which 192 were similar in both strains. Only five proteins were exclusive to the ICC-45 strain. These proteins were involved with catalytic and binding functions and indirectly interacted with proteins related to pathogenicity. Most proteins, including TcdA, TcdB, flagellin subunit, and cell surface protein, were overrepresented in the ICC-45 strain; 14 proteins, including mature S-layer protein, were present in higher proportions in LIBA5756. Data are available via ProteomeXchange with identifier PXD026218. These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains.
Highlights
Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America
In the early 2000s, the epidemiology of C. difficile infection (CDI) drastically changed with the emergence of BI/NAP1/ribotype 027, an epidemic and hypervirulent strain
The aim of this study was to use a proteomic and genomic approach to compare two multilocus sequence type (MLST) clade 2 C. difficile strains with hypervirulent features, ICC-45 (ST41)[9] and NAP1/RT027 (ST01)[2,9], isolated in Brazil and Costa Rica, respectively
Summary
Clostridioides difficile BI/NAP1/ribotype 027 is an epidemic hypervirulent strain found worldwide, including in Latin America. Data are available via ProteomeXchange with identifier PXD026218 These data show close similarity between the genome and proteins in the supernatant of two strains with hypervirulent features isolated in Latin America and underscore the importance of epidemiological surveillance of the transmission and emergence of new strains. A recent study compared whole-genome sequences of 25 NAP1, RT027, or ST01 C. difficile clinical isolates with 129 isolates from the same genotype collected worldwide These lineages entered Mexico, Costa Rica, Honduras, and Chile from different geographic areas, suggesting that the B1/NAP1/RT027/ST01 isolates from these countries are susceptible to acquiring distinct single-nucleotide polymorphisms and genes implicated in antibiotic r esistance[5]. The epidemic strain, NAP1/027, had not been isolated in Brazil to date; clade 2 strains have been isolated in two locations, including the one analyzed here
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