Exopolysaccharides produced by Lactobacillus strains suppress HT-29 cell growth via induction of G0/G1 cell cycle arrest and apoptosis.

Abstract

In the present study, the effects of exopolysaccharides (EPS) from nine Lactobacillus strains with a high degree of bio-activity on human colon cancer cell line HT-29 were studied. The extracellular polymeric substances from 4 strains, namely K11, M5, SB27 and ×12, displayed desirable anti-proliferative activity against HT-29 cells. Crude and acidic EPS were purified from the 4 strains and the inhibitory effects were further investigated. The crude and acidic EPS from these 4 strains exerted anti-proliferation effects on HT-29 cells in a dose-dependent manner but were nontoxic to Vero cells. Treatment with EPS significantly induced G0/G1 cell cycle arrest and apoptosis of HT-29 cells. Hoechst 33258 staining of acidic EPS-treated HT-29 cells revealed different degrees of morphological changes within the nucleus and the formation of apoptotic bodies. Caspase-3 activity was markedly upregulated in HT-29 cells following treatment with acidic EPS. In addition, acidic EPS from the SB27 strain demonstrated the most robust inhibitory effect on HT-29 cells. The results of the present study suggest that as an inducer of apoptosis EPS has the potential to be applied in the treatment of colorectal cancer.