Exopolysaccharides from Lactobacillus plantarum reduces cadmium uptake and mitigates cadmium toxicity in rice seedlings.

Abstract

Exopolysaccharides (EPSs) can be used as effective exogenous substances to alleviate the toxic effect of cadmium (Cd) on rice and other crops, thus improving plant growth characteristics under stress conditions, and reducing the accumulation of Cd in grains, but the underlying mechanism is still unclear. In the present work, the effects of EPSs from Lactobacillus plantarum on the efficiency of Cd absorption and distribution in rice seedlings under Cd stress were investigated. The results revealed that growth of rice seedlings was severely inhibited by exposure to Cd, resulting in the decrease of plant height, leaf length and biomass. This inhibition phenomenon was alleviated by the addition of EPSs from L. plantarum LPC-1. The underlying mechanism might be that EPSs could facilitate the accumulation efficiency of Cd in rice roots and reduce the transportation rate of Cd from root to leaves, therefore decreasing the Cd content in leaves. Further research showed that Cd contents in the cell wall fraction of the rice seedling root were increased by the addition of EPSs, while the proportions of Cd in the cell organelle and cell soluble component were reduced. Application of EPSs promotes the proportion of pectate- and protein- integrated Cd in rice roots. While the content of water-soluble Cd, which is more toxic to plants, decreased continuously both in roots and leaves. Our study clearly confirmed the positive effects of EPSs on alleviating Cd toxicity and decreasing Cd translocation in rice above-ground parts. Furthermore, the subcellular distribution and chemical forms of Cd in different rice seedlings parts were also affected by the addition of EPSs, which might be an important potential mechanism for EPSs in respect of alleviating Cd toxicity for rice. These findings provided a foundation for the application of exogenous substances on improving the growth performance of crops under heavy metal stress.