Abstract
The length of the terminal repetition of herpes simplex virus (HSV) DNA has been measured by electron microscopic observation of the overlap regions of the circular molecules formed by annealing the single-stranded DNA ends exposed after limited exonuclease III digestion. The terminal repetition was found to be 1560 ± 400 base pairs (1.0% of the total length of HSV DNA) on each end of the DNA. This value is twice the previously published value; experiments to explain this possible discrepancy have been undertaken. It has also been found that HSV DNA purified from the Hirt supernatant of virus-infected cells by preparative glycerol gradient sedimentation followed by CsCl buoyant density centrifugation could not be digested with exonuclease III sufficiently to produce circles unles the HSV DNA was subjected to additional rigorous deproteinization. In contrast, λ-exonuclease would digest HSV DNA sufficiently to produce circles both before and after rigorous deproteinization. These data are taken as preliminary evidence for a protein bound at or near the 3′-hydroxyl terminus of HSV DNA.
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