Exon trapping for positional cloning and fingerprinting.

Abstract

AbstractPositional cloning involves the genetic, physical, and transcript mapping of specific parts of a genome (1). Linkage analysis can map specific activities, or phenotypes, to a quantitative trait locus (QTL), a genomic region no smaller than 1 centiMorgan (cM) or megabase (Mb) in length. Physical mapping can then provide a map of higher resolution. Physical maps are constructed from clones identified by screening genomic libraries. Genomic clones can be characterized by fingerprinting and ordered to create a contig, a contiguous array of overlapping clones. Transcript identification from the clones in the contig results in a map of genes within the physical map. Finally, expressional and functional studies must be performed to verify gene content.KeywordsQuantitative Trait LocusCryptic Splice SiteConsensus Splice SiteExon TrappingTranscript IdentificationThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.