Exogenously-applied 5-aminolevulinic acid modulates some key physiological characteristics and antioxidative defense system in spring wheat (Triticum aestivum L.) seedlings under water stress

Abstract

Aminolevulinic acid, one of the key precursors involved in chlorophyll biosynthesis, was applied exogenously to assess its role in improving seedling drought stress tolerance in two wheat cultivars (Shafaq-2000 and Auqab-06). Varying levels (0, 50, 100 and 150mgL−1) of 5-ALA were foliarly applied to one month old seedlings. Data showed that plant growth (shoot and root fresh and dry weights), chlorophyll a and b contents, chlorophyll a/b ratios, and leaf and root P of seedlings of both wheat cultivars decreased considerably at both drought stress regimes i.e. 80% and 60% of field capacities. In contrast, water-use efficiency (WUE) calculated as A/E and proline contents accumulated considerably under drought conditions in both wheat cultivars. However, none of the two water regimes altered sub-stomatal CO2 concentration (Ci), photosynthetic rate (A), stomatal conductance (gs), transpiration rate (E) and Ci/Ca ratio (Ca; ambient CO2 concentration), glycine betaine (GB) contents, leaf and root N, Ca2+, and K+ concentrations, activities of enzymatic antioxidants such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and total soluble proteins in the seedlings of both wheat cultivars. 5-ALA enhanced shoot and root fresh and dry weights, chlorophyll pigments, GB contents, root K+, and N (leaf & root) in both wheat cultivars at the different water stress regimes. Of all ALA levels, 50 and 100mgL−1 were most effective in improving seedling growth. However, shoot and root fresh weights, shoot dry weights, chlorophyll a and b contents, A, E, gs, Ci and Ci/Ca, and proline contents were not affected by ALA treatments. Of the wheat cultivars, cv. Shafaq-2000 showed significantly higher shoot fresh and dry weights, chlorophyll b contents, gs and Ci, while cv. Uqab-06 was relatively better at proline accumulation, leaf and root P and root N concentrations. Overall, foliar-applied 5-ALA improved growth, chlorophyll a and b contents, GB, root K+, leaf and root N contents in both wheat cultivars at different water stress regimes, while all other attributes were not affected significantly.