Abstract
Gibberellin A4 (GA4) is biologically active in Salix pentandra and is able to induce stem elongation in seedlings grown under short day (SD) conditions, as well as in seedlings grown under long day (LD) conditions and treated with a growth retardant BX-112. [3H2]GA4 and [2H2]GA4 were applied to seedlings and leaf and stem explants of S. pentandra, and metabolites were studied using HPLC and GC-MS. After application of [3H2]GA4 to seedlings of S. pentandra, one of the three main radioactive metabolites in the free acid fraction had retention properties similar to GA1. Using [2H2]GA4, this compound was identified by GC-MS with SIM as [2H2]GA1 both from short day-grown and BX-112-treated seedlings, as well as in leaf and stem explants. After injection of GA4 into a mature leaf, GA1 was mainly found in the elongating stem tissue. Thus, the possibility that the biological activity of GA4 in Salix is due to its conversion to GA1 cannot be excluded.
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