Abstract
Spermidine is important for the hypothalamic control of pituitary secretion of hormones involved in neuroendocrine functions in mammals. In this study, the effect of exogenous spermidine on the expression of genes and proteins related to polyamine metabolism and polyamine levels was examined. The results indicated that treatment with spermidine at 0.05 mg/g (BW) significantly increased the levels of Oaz1 mRNA and protein expression and decreased putrescine content in mouse hypothalamus (p < 0.05). The administration with spermidine at 0.10 mg/g significantly increased the levels of Oaz1, Oaz2, and Odc expression in mouse hypothalamus (p < 0.05). Treatment with spermidine at 0.05 mg/g significantly increased the levels of Ssat mRNA expression and reduced the level of Smo mRNA expression in mouse hypothalamus (p < 0.05). Putrescine concentrations in the hypothalamus after the administration of spermidine at 0.10 and 0.15 mg/g were significantly higher than those in the control group (p < 0.05). The concentration of both spermidine and spermine in the hypothalamus after the administration of spermidine at 0.15 mg/g was decreased significantly (p < 0.05). In summary, our results indicate that exogenous spermidine affects polyamine homeostasis in the mouse hypothalamus by modulating the expression of genes and proteins related to polyamine metabolism.
Highlights
Spermidine is important for the hypothalamic control of pituitary secretion of hormones involved in neuroendocrine functions in mammals
The level of OAZ1 protein in the mouse hypothalamus after the administration of spermidine at 0.05 mg/g was 1.49fold compared to the control group (p < 0.05), while the level of OAZ1 protein in mice treated with 0.10 and 0.15 mg/g spermidine was not significantly different compared to the control group (p > 0.05) (Figure 3)
There was no significant difference in the mRNA expression levels of Azin1, Azin2, Samdc, Spds, and Spms in the hypothalamus of mice treated with spermidine compared to control mice (p > 0.05) (Figure 1)
Summary
Abstract: Spermidine is important for the hypothalamic control of pituitary secretion of hormones involved in neuroendocrine functions in mammals. The results indicated that treatment with spermidine at 0.05 mg/g (BW) significantly increased the levels of Oaz mRNA and protein expression and decreased putrescine content in mouse hypothalamus (p < 0.05). Our results indicate that exogenous spermidine affects polyamine homeostasis in the mouse hypothalamus by modulating the expression of genes and proteins related to polyamine metabolism. Thyssen and Libertun [11] reported that spermidine level was high during the first postnatal days in the rat hypothalamus, putrescine reached the highest concentration on postnatal day 6, and α-difluoromethylornithine (a specific and irreversible inhibitor of ODC) decreased putrescine and spermidine levels in the hypothalamus These results indicate that spermidine is important for the hypothalamic control of. Our results demonstrated that spermidine affected polyamine metabolism in the mouse hypothalamus by modulating the expression of genes and proteins related to polyamine metabolism
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