Exogenous proline enhances salt tolerance in acclimated Aloe vera by modulating photosystem II efficiency and antioxidant defense

Abstract

This study investigated the ameliorating effect of exogenous proline on some physiological responses of salt acclimated and non-acclimated Aloe vera plants against subsequent salt stress (EC 21 dS/m). Compared to control, salinity decreased leaf growth and relative water content (RWC), leaf contents of chlorophyll a, carotenoids, K/Na ratio, photosystem performance index (PIabs), while promoted leaf contents of hydrogen peroxide (H2O2), maloendialdehyde (MDA) and Na, which subsequently induced oxidative stress. However, foliar applied proline was effective in alleviating the deleterious effects of salinity. Maximum amelioration of salt stress by exogenous proline was found only in salt-acclimated plants, which was attributed to the reduced leaf Na accumulation together with enhanced K content. Proline pretreatment improved plant relative water content, which was correlated with enhancement in leaf proline concentration. We showed that application of proline on salt-acclimated stressed plants alleviated the damage to PSII functioning induced by salinity, contributing to the improvement of the oxygen-evolving complex efficiency of PSII (Fv/Fo) and the quantum yield of electron transport (ΦEo). This improvement of PSII activity was achieved by higher carotenoids and phenolic accumulation. Additionally, proline treatment reduced the accumulation of H2O2 and MDA, indicating that proline pretreatment was more effective in lowering oxidative damage in salt-exposed acclimated plants, which was associated with the activation of SOD, CAT and APX enzymes. Thus, proline pretreatment could protect salt acclimated Alo vera plants against subsequent salt stress through improving K/Na ratio and water relations attributes as well as enhancing antioxidant systems, resulting in better photosynthesis under salt stress.