Exogenous Oxalic Acid and Citric Acid Improve Lead (Pb) Tolerance of Larix olgensis A. Henry Seedlings

Jinfeng Song,Chengwei Duan,Shaoping Wu,Xiaoyang Cui,Ying Sang,Daniel Markewitz

doi:10.3390/f9090510

Jinfeng Song, Chengwei Duan + Show 4 more

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https://doi.org/10.3390/f9090510

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Journal: Forests	Publication Date: Aug 23, 2018
Citations: 22	License type: CC BY 4.0

Affiliation: Northeast Forestry University, University of Georgia

Abstract

We investigated the beneficial role of different concentrations of exogenous oxalic acid (OA) or citric acid (CA) for improving Pb tolerance and mitigating Pb-induced physiological toxicity in Changbai larch (Larix olgensis A. Henry) seedlings in northeast China. The seedlings were exposed to 100 mg·kg−1 Pb in soil alone or in combination with OA or CA irrigation for 10, 20, or 30 days. Pb-induced damage in L. olgensis was evident from elevated lipid peroxidation that significantly inhibited plant growth. Malondialdehyde (MDA) contents also increased in the presence of elevated Pb; however, superoxide dismutase (SOD) and peroxidase (POD) activities, as well as proline and pigment contents, all decreased. The damage increased in controls over the application periods. Pb contents in fine roots and leaves generally decreased with low-concentration organic acids (<1.0 mmol·L−1), but often increased at 5.0 and 10.0 mmol·L−1. Alternatively, when Pb-stressed plants were exposed to an organic acid (especially 5.0 or 10.0 mmol·L−1 for 10 days), the damage, as indicated by the physiological parameters, was reversed, and plant growth was promoted; CA was more effective in inducing these changes than OA. Therefore, exogenous organic acids have the potential to alleviate Pb-induced oxidative injuries, and can improve the tolerance of L. olgensis seedlings to Pb stress. Under lower OA and CA concentrations, the detoxification mechanism appears to be an external resistance mechanism; however, under higher concentrations (5.0–10.0 mmol·L−1) internal resistance mechanisms appear dominant. It is also possible that the two mechanisms work in tandem.

Highlights

Heavy-metal pollution has become a growing environmental problem in many countries around the world due to anthropogenic activities such as mining and smelting, the excessive use of chemical fertilizers, long-term wastewater irrigation, and vehicle emissions [1]
We found support for our hypothesis that exogenous organic acids may protect L. olgensis seedlings against Pb toxicity, as evidenced by the reversal in physiological characteristics indicating seedlings against Pb toxicity, as evidenced by the reversal in physiological characteristics indicating stress
The growth of seedling height, belowground biomass, POD activities, proline and carotenoid contents in absolute growth of seedling height, belowground biomass, POD activities, proline and carotenoid leaves, and Pb contents in fine roots and leaves, the effects of citric acid (CA) were all greater than oxalic acid (OA)

Summary

Introduction

Heavy-metal pollution has become a growing environmental problem in many countries around the world due to anthropogenic activities such as mining and smelting, the excessive use of chemical fertilizers, long-term wastewater irrigation, and vehicle emissions [1]. This pollution resulted in soil contamination that is a hazard to human health through soil or edible plants. Its high toxicity affects plant physiological processes and growth, both of which are related to negative effects on nutrient uptake, respiration, photosynthesis, and antioxidant enzymes [3].

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