Exogenous Mouse Mammary Tumor Virus (MMTV) Infection Induces Endogenous MMTVsagExpression

Abstract

The mouse mammary tumor virus (MMTV) superantigen (Sag) protein is involved in the transmission of milk-borne MMTV from virus-infected milk in the gut to the target mammary gland tissue. Using an RT–PCR assay forin vivoMMTV infection, BALB/c or C3H mice nursed on C3H MMTV-infected mothers showedsagmRNA expression in intestine, spleen, and thymus as early as 1 day after infection, whereas uninfected BALB/c control animals had approximately 10- to 30-fold lowersagexpression. Further fractionation experiments with small intestine indicated thatsagexpression occurred in gut-associated lymphoid cells. Restriction enzyme digestion of PCR products indicated that thesagmRNA detected was derived from the endogenous MMTVs, and sequencing analysis confirmed that the PCR products were derived from endogenousMtv-6.Expression of C3H-specific mRNA was detectable in BALB/cfC3H or C3H tissues by RNase protection or by RT–PCR. Endogenous MMTVsagexpression was low in spleen and undetectable in thymocytes of C3H MMTV-infected C57BL/6 mice, a strain resistant to C3H MMTV tumorigenesis and defective for MHC class II I-E molecules. The RT–PCR assay forsagmRNA appears to measure the Sag-induced stimulation previously predicted for milk-borne MMTV infection. Together these data suggest that exogenous MMTVsagexpression is minimal, but sufficient to rapidly stimulate transcription of endogenous MMTVsagmRNA in B- and T-cells in an MHC class II I-E-dependent manner. The endogenoussagexpression on maternal lymphocytes may increase the number of proliferating T-cells available for milk-borne MMTV infection.