Exogenous <i>Cry1Ac/CpTI </i>of Common Wild Rice by Introgressing from Transgenic Cultivated Rice can be Stable Inheritance and Expression

Abstract

With the rapidly development of transgenic rice and increasing environmental release, escape of foreign genes through pollen into wild relatives happens possible, the potential ecological effects may become more and more attention. In this study, Cry1Ac/CpTI bivalent transgenic insect-resistant genes from cultivated rice introgressing into common wild rice ( Oryza rufipogon Griff) were carried out to generate F 1 and their offspring of F 2 , F 3 , F 4 and F 5 , as well as backcross progenies, BC 1 F 1 and BC 1 F 2 , and also inheritance and expression of foreign genes in wild rice offspring were analyzed in order to clarify whether or not escape of foreign genes from the transgenic cultivated rice might be stable inheritance and expression in the wild relatives of rice. The results showed that exogenous gene copy number in the wild rice offspring was completely identical to the gene donor of cultivated rice. The insertion loci of foreign genes in common wild rice were also quite stable. The expression patterns and expression levels of insecticidal protein Cry1Ac in the different generations of wild rice were almost consistent to that of their gene donor of cultivated rice. This study illustrated that foreign genes of transgenic rice once escaping into wild relatives might be introgressing into other rice cultivar and wild relatives of rice, The foreign genes can be stable inherited and expressed, which imply that GM rice escape of foreign genes from GM rice into closely related species might exist potential ecological risk.