Exogenous Hydrogen Peroxide Changes Antioxidant Enzyme Activity and Protects Ultrastructure in Leaves of Two Cucumber Ecotypes Under Osmotic Stress

Abstract

Cucumber (Cucumis sativus L.) varieties cv. Jinchun no. 4 (a North China ecotype) and cv. Lvfeng no. 6 (a South China ecotype) were cultivated to explore the effects of osmotic stress on the ultrastructure of chloroplasts and mitochondria, as well as to assess the possible protective effect of exogenous hydrogen peroxide (H2O2). Under osmotic stress induced by 10% polyethylene glycol 6000, 84.3% of the chloroplasts in Jinchun no. 4 were abnormal, whereas 88.6% were abnormal in Lvfeng no. 6. Abnormal mitochondria occurred in these two strains at rates of 78.5 and 87.1%, respectively. The stress condition disintegrated the membranes of most chloroplasts and mitochondria in the leaf cells of both cucumber ecotypes, and it also increased the malondialdehyde (MDA) content. We subjected the two cultivars to a combined treatment with H2O2 and osmotic stress and made the following observations: (1) Abnormal chloroplasts occurred at rates of 25.7 and 28.6%, and abnormal mitochondria were observed at rates of 22.9 and 32.8%, respectively. (2) Most of the investigated membranes were well organized in leaves of Jinchun no. 4 and Lvfeng no. 6, and the levels of endogenous H2O2, superoxide anion, and MDA were lower. Osmotic stress and exogenous H2O2 both increased the activities of antioxidative enzymes such as manganese superoxide dismutase, glutathione peroxidase, catalase, guaiacol peroxidase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, dehydroascorbate reductase, and the antioxidants ascorbate and reduced glutathione. The combined effect of osmotic stress and exogenous H2O2 resulted in the highest antioxidant activities in both cucumber ecotypes. We propose that exogenous H2O2 increases antioxidant activity in cucumber leaves and thereby decreases lipid peroxidation to some extent, thus protecting the ultrastructure of most chloroplasts and mitochondria under osmotic stress.