Abstract
Two experiments were carried out to investigate the effects of feeding diets containing faba beans and peas on the concentration, flow and composition of total, protein-bound and free amino acids (AA) in pancreatic juice collected from young pigs fitted with a pancreatic pouch re-entrant cannula. In Experiment 1, eight barrows, with an average initial weight of 8.5 kg, were fed one of two semi-purified diets formulated to contain 200 g kg -1 crude protein (CP) according to a completely randomised design. In one of the diets, soya bean meal was the sole protein source ; in the other diet, soya bean meal and faba beans (cv Fibro ; dark-flowering) each supplied 50% of the dietary CP. In Experiment 2, five barrows, with an average initial weight of 18.1 kg, were fed one of two diets formulated to contain 150 g kg -1 CP with peas (cv Ascona or cv Radley) as the sole protein source. There was no effect (P > 0.05) of the diet, 8 h sampling period or experimental period on the concentration (mM), composition (% and g 16 g N -1 ) and flow (mmol 24 h -1 ) of total, protein-bound and free AA or on the percentage of protein-bound AA in pancreatic juice. The concentrations of total, protein-bound and free AA were higher in pancreatic juice collected from younger pigs in Experiment 1 than in pancreatic juice collected from older pigs in Experiment 2. However, the daily flows of total, protein-bound and free AA in pancreatic juice were similar in both experiments. The percentages of protein-bound AA in pancreatic juice were similar in both experiments. In conclusion, the concentrations, compositions and flows of total, protein-bound and free AA in pancreatic juice were not affected by diet, 8 h sampling period or experimental period.
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