Exocrine Pancreatic Secretions in Growing Pigs Fed Diets Containing Fish Oil, Rapeseed Oil or Coconut Oil

Abstract

Two experiments were performed to study the effect of feeding diets containing oils with different fatty acid composition on exocrine pancreatic secretions in growing pigs using two different methods to collect pancreatic juice. In the first experiment, three barrows (initial weight 37 kg) were fitted with a pancreatic pouch re-entrant cannula. An isolated pouch was prepared where the pancreatic duct enters the duodenum. In the second experiment, also using three barrows (initial weight 32 kg), a catheter was inserted into the pancreatic duct. Three wheat starch and fish meal-based diets were formulated to contain either 15 g fish oil, rapeseed oil or coconut oil/100 g. In both experiments, the diets were fed according to a 3 times 3 Latin square design. The volume of pancreatic juice secreted, pH and secretion of bicarbonate, protein, amylase, trypsin, lipase and colipase were not significantly affected by the diets in the first experiment. In the second experiment, chymotrypsin secretion was significantly greater in pigs fed the coconut oil diet, and secretion of carboxyl ester hydrolase was significantly higher in pigs fed the fish oil diet. When compared qualitatively, pigs in Experiment 2 secreted more pancreatic juice; the pancreatic juice had a higher pH, and trypsin, carboxyl ester hydrolase and colipase secretions were substantially higher whereas amylase secretion was lower than for pigs in Experiment 1. The fatty acid composition of the different oils had minor effects on exocrine pancreatic secretion in growing pigs. However, there were considerable differences between the two surgical methods used to collect pancreatic juice, and these differences may be explained by physiological changes induced by the two methods.