Abstract
Diglycolic acid dehydrogenase activity linked with 2,6-dichlorophenolindophenol and phenazine methosulfate was found in the particulate fraction of the cell-free extract of a mixed culture of Flavobacterium and Pseudomonas species grown on polyethylene glycol 6000. The amount of glyoxylic acid formed increased with the increase in reaction time and enzyme concentration. Horse heart cytochrome c, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl 2H-tetrazolium bromide, and nitro blue tetrazolium, served as hydrogen acceptors in the presence of phenazine methosulfate. Enzyme activity was competitively inhibited by 1,4-benzoquinone. The enzyme was also active on tetraethylene glycol dicarboxylic acid, a metabolite of tetraethylene glycol, and on methoxy- or ethoxyacetic acid.
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