Abstract
Abstract The effect of various substances has been studied on the secretion of chitinase (chitin glycanohydrolase, EC 3.2.1.14) by the isolated gastric mucosa of Reptilia and Amphibia. Our results show that N -methylpyridinium-2-aldoxime iodide, the antidiuretic hormone, glycine and DNP are without effect on the secretory process. The electrical potential difference existing spontaneously between the two faces of the epithelium is also unaffected except in the case of DNP which abolishes it. On the contrary, monoiodoacetate and sulfate ions inhibit the secretion of chitinase, and remove the electrical potential difference. In the presence of sulfate ions the potential difference is sometimes reversed. When the potential difference is short-circuited by means of an external electromotive force, the secretion is not modified. Consequently, there is no direct relationship between potential difference and chitinase secretion. One way of explaining the effect of monoiodoacetate is to postulate an action on the sulfhydryl group of the membrane. From our results it is reasonable to suggest that the secretion of chitinase depends on a mechanism involving the formation of a complex between the enzyme and some structural element of the membrane.
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