Exerting applied voltage promotes microbial activity of marine anammox bacteria for nitrogen removal in saline wastewater treatment

Abstract

To date, the application of marine anammox bacteria (MAB) is still a challenge in saline wastewater treatment due to the low growth rate and high sensitivity. Herein, bioelectrochemical system with applied voltage was exerted for the first time to promote the activity of MAB for removing nitrogen from saline wastewater. At the optimal voltage of 1.5 V, the mean total nitrogen removal rate (TNRR) reached the maximum of 0.65 kg/m3•d, which was 27.45% higher than that without applied voltage. Besides, applied voltage reduced the microbial diversity of MAB-based consortia, but the relative abundance of Candidatus Scalindua increased by 4.63% at 1.5 V compared with that without applied voltage. Also, proper applied voltage promoted the secretion of EPS and heme c, which resulted in the enhancement of MAB activity. Based on the remodified Logistic model analysis, the lag time of the nitrogen removal process was shortened by 0.72 h at the voltage of 1.5 V. Furthermore, it was found that higher voltage (> 2.0 V) had a negative effect on the MAB activity for low TNRR of 0.33 kg/m3•d (2.5 V). However, TNRR increased back to 0.61 kg/m3•d after removing the high applied voltage, which implied that the bioactivity was recoverable after being inhibited. These findings demonstrated that external electrical stimulation is an effective strategy to promote nitrogen removal and MAB activity for treating saline wastewater.