Abstract
Our aim was to explore the effects of dietary and behavior interventions on lipometabolism caused by an unhealthy high-fat diet and the best method to rebuild lipid homeostasis of this lifestyle. Apart from normal diet rats, 34 rats were fed with high-fat emulsion for 4 weeks and then intervened for another 4 weeks. Eight of them were classified into high-fat control group, and 9 were sorted into high-fat diet with rice vinegar group. Meanwhile, 10 were put into high-fat diet in swimming group, and 7 were just for refeeding normal diet group. Then, the data of body weight was recorded and analyzed. Indexes of serum samples were tested by kits. AMPKα, HNF1α, and CTRP6 in pancreas, liver, cardiac, and epididymis adipose tissues were detected by western blot. According to our experiments, swimming and refeeding groups reflected a better regulation on lipid homeostasis mainly by upregulating the expression of pancreas AMPKα. To be more specific, the refeeding rats showed lower T-CHO (P < 0.001) and LDL-C (P < 0.05), but higher weight gain (P < 0.001), insulin level (P < 0.01), and pancreas AMPKα (P < 0.01) than high-fat control rats. Compared with rats intervened by swimming or rice vinegar, they showed higher weight gain (P < 0.001), insulin level (P < 0.01), and HNF1α, but lower of CTRP6. In summary, refeeding diet functioned better in regulating the lipometabolic level after high-fat diet. Whatever approach mentioned above we adopted to intervene, the best policy to keep the balance of lipid homeostasis is to maintain a healthy diet.
Highlights
High-fat diet is the main cause of abnormal lipometabolism, such as hyperlipidemia, obesity, and cardiovascular disease
We demonstrated different interventions by rice vinegar, swimming, and refeeding to high-fat diet rats might lead to discrepant effects
The results of refeeding showed, there was no function of losing weight in refeeding rats, the content of T-CHO and LDL-C, insulin level, and AMPKα expression in pancreas and liver tissues were regulated significantly
Summary
High-fat diet is the main cause of abnormal lipometabolism, such as hyperlipidemia, obesity, and cardiovascular disease. Regulating the balance of lipid intake and exclusion is essential to maintain lipid metabolism. Excessive intake of lipids from diet affects the flux of substrates through lipogenesis and lipid oxidation to intervene related metabolism and insulin biological function [2]. In order to elaborate the mechanisms of disordered lipid metabolism, different highfat diet rat models have been investigated by studies. Rats fed with a high-fat diet for 7 weeks were diagnosed with obvious hyperlipidemia and fatty liver [3]; 8 weeks of high-fat diet might lead to significantly increased body weight and lipid and glucose abnormalities in the rat models of hyperlipidemia and hyperglycemia [4]. Disordered lipid metabolism caused by high-fat diet intervention is one of the classical methods in the metabolic field
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