Exercise Induce HO-1 Expression in Skeletal Muscle and Improve Sports Ability of Mice

Abstract

Abstract:Aim:We aimed to determine the effects of exhaustion exercise on HO-1 expression, the function of improving the sports ability and promoting the exercise induced fatigue in mice.Methods:Breeding, adaptively exercise BDF1 mice, 6 weeks age.They were randomly divided into normal saline control group,zinc protoporphyrin pharmacological inhibition group, the cobalt protoporphyrin drug induced group, HO-1 genetically modified group, each group was used for exhaustion exercise on the treadmill, and divided into immediate group and 24h after exhaustion groups.HO-1mRNA and protein, serum CK, BUN, urine protein were measured by using RT-PCR,Western-blot method, kinetic method, two point method and coomassie brilliant blue method. Results:The results indicated that HO-1mRNA expression increased after exhaustion exercise in the salinegroup, the other three groups changed little, HO-1 protein after exhaustion exercise of the saline group and drug inhibition group was higher than pre-exercise,24h after exercise obviously declined,24h after exhaustion exercise in drug induced group was higher than pre-exercise than immediately exercise.Exhaustion exercise time of the drug induced group(383.08±65.04min) and genetically modified mice(468.25±52.41min)was longer than the pharmacological inhibition (341.08±73.03min)and saline group(468.25±52.41min),significant variations were found in them(p<0.05). CK, BUN and urine protein removal were faster in drug induced group, the pharmacological inhibition of the group was slower. CK, BUN were faster in genetically modified group, but urine protein was in a higher level. Conclusion:Exhaustion exercise can induce skeletal muscle HO-1 expression of the wild type mice, it is not obvious in the higher HO-1 expression mice. Expression of HO-1 must be within limits, which can enhance mice exercise ability and promote the recovery of exercise fatigue.