Abstract
Background Endothelial dysfunction commonly occurs in obese children and adolescents, leading to an increased risk of cardiovascular diseases. Exercise has significant protective effects against endothelial dysfunction through regulating some noncoding RNAs. This study aimed to investigate the relationship of long noncoding RNA MALAT1 and microRNA-320a (miR-320a) with the exercise-induced improvement of endothelial dysfunction in obese children and adolescents. Methods Sixty obese children and adolescents were included in this study, including 40 cases that received 12-week exercise training and 20 cases that received only diet control. The anthropometric and blood indices before and after exercise were recorded and compared, and the endothelial dysfunction was evaluated by examining the levels of markers, including VCAM-1, ICAM-1, and E-selectin, using an ELISA assay. The expression levels of noncoding RNAs were assessed using real-time quantitative PCR, and their correlation with patients' recorded indices and endothelial dysfunction markers was analyzed. Results The 12-week exercise training significantly decreased the levels of VCAM-1, ICAM-1, and E-selectin and could inhibit MALAT1 but promote miR-320a expression in obese children and adolescents. The expression of MALAT1 and miR-320a was correlated with the changes in the anthropometric and blood indices of obese children and adolescents, and their correlations with endothelial dysfunction markers were obtained. Conclusion All the data revealed that exercise has significantly protective effects against endothelial dysfunction and can regulate the expression of the MALAT1/miR-320a axis. MALAT1 and miR-320a were correlated with endothelial dysfunction markers, indicating that the MALAT1/miR-320a axis may be related with the alleviating effects of exercise on endothelial function in obese children and adolescents.
Highlights
Obesity is a disease in which long-term energy intake exceeds consumption, resulting in excessive energy stored in the form of fat and increased risk of diseases in various systems of the body [1]. us, obesity becomes one of the global public health burdens in both developed and developing countries, affecting the health and life quality of both adults and children [2]
Previous studies have reported that the levels of reactive oxygen species (ROS), C-reactive protein (CRP), and proinflammatory cytokines were significantly increased when endothelial dysfunction occurs, which led to the enhanced expression and secretion of some adhesion molecules, such as intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1), and E, L, and P-selections, and promoting the concentration of blood and the formation of small patches [6, 7]
The expression levels of MALTA1 and miR320a in obese children and adolescents before and after exercise were examined, and their correlation with endothelial dysfunction was investigated by evaluating the levels of VCAM-1, ICAM-1, and E-selectin. e findings of this study may uncover the relationship between the Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1)/ miR-320a axis and the improved endothelial function induced by exercise
Summary
Endothelial dysfunction commonly occurs in obese children and adolescents, leading to an increased risk of cardiovascular diseases. E anthropometric and blood indices before and after exercise were recorded and compared, and the endothelial dysfunction was evaluated by examining the levels of markers, including VCAM-1, ICAM-1, and E-selectin, using an ELISA assay. E expression levels of noncoding RNAs were assessed using real-time quantitative PCR, and their correlation with patients’ recorded indices and endothelial dysfunction markers was analyzed. E 12-week exercise training significantly decreased the levels of VCAM-1, ICAM-1, and E-selectin and could inhibit MALAT1 but promote miR-320a expression in obese children and adolescents. E expression of MALAT1 and miR-320a was correlated with the changes in the anthropometric and blood indices of obese children and adolescents, and their correlations with endothelial dysfunction markers were obtained. All the data revealed that exercise has significantly protective effects against endothelial dysfunction and can regulate the expression of the MALAT1/miR-320a axis. MALAT1 and miR-320a were correlated with endothelial dysfunction markers, indicating that the MALAT1/miR-320a axis may be related with the alleviating effects of exercise on endothelial function in obese children and adolescents
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