Abstract
Zygosaccharomyces soja mutant obtained from cycloheximide treatment accumulated several kinds of nucleotides together with riboflavin in the minimal glucose salt medium. Accumulation of nucleotides took place in parallel with the propagation of the microorganism after inoculation. That the nucleotides accumulated were identical to 5′-CMP, 5′-AMP, 5′-UMP, 5′-GMP and UDP, ADP, GDP respectively was verified using chemical and enzymic procedures. It was assumed that the excretion of these nucleotides was mainly carried out by the action of polynucleotide phosphorylase.It was also surmised that the excretion of AMP at the early stage of incubation meant leakage from de novo synthesis.
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