Excretion of primary prostanoids and their metabolites during acute volume expansion

Abstract

Simultaneous determination of urinary excretion rates of primary unmetabolized prostanoids and their enzymatic metabolites were performed by gas chromatography-mass spectrometry (GC/MS) or tandem mass spectrometry (GC/MS/MS). Changes in kidney function were induced by acute (4 h) volume expansion. Despite marked changes in urine flow, GFR, urinary pH, osmolality, sodium and potassium excretion, only a insignificant or transient rise in the enzymatic prostanoid metabolites (2,3-dinor-6-keto-PGF 1α, PGE-M, 2,3-dinor-TxB 2 and 11-dehydro-TxB 2) was observed. The excretion rates of the primary prostanoids were elevated in parallel with the rise in urine flow: PGE 2 rose (p < 0.05) from 14.2 ± 4.0 to 86.2 ± 20.7, PGF 2 α from 60.0 ± 4.9 to 119.8 ± 24.0, 6-keto-PGF 2α from 7.2 ± 1.3 to 51.5 ± 17.0, and txB 2 from 11.2 ± 3.3 to 13.6 ± 3.6 ng/h/1.73 m 2 ( x ̄ ± SEM ) at the maximal urine flow. Except for 6-keto-PGF 1α and TxB 2, this rise in urinary prostanoid levels was only transient despite a sustained fourfold elevated urine flow. We conclude that urine flow rate acutely affect urine prostanoid excretion rates, however, over a prolonged peroid of time these effects are not maintained. The present data support the concept that urinary levels of primary prostanoids mainly reflect renal concentrations whereas those of enzymatic metabolites reflect systemic prostanoid activity. From the excretion pattern of TxB 2 one can assume that this prostanoid represents renal as well as systemic TxA 2 activity.