Excretion of Bromsulfalein in the Bile

Abstract

Bromsulfalein, injected intravenously in a dosage of 2 mg per kilo of body weight, is rapidly removed from the blood stream (85-95% in 5 minutes; 100% in 30 minutes), but its elimination in the bile continues over a period of several hours. This suggests that two separate or related mechanisms are involved and that simultaneous investigation of the rapidity of removal of the dye from the blood and the curve of its elimination in the bile might yield information of interest.The concentration of bromsulfalein in the bile was determined as follows: 0.1 cc of bile was added to 15 cc of distilled water and 0.1 cc of 10% NaOH, and mixed by inversion. A blank was prepared for each specimen, containing 0.1 cc of bile, 15 cc of water and 0.1 cc of 10% NaCl. If turbidity developed the mixtures were filtered. After 10 minutes, readings were made in the Evelyn photoelectric colorimeter, using filter 580, and the concentration of bromsulfalein determined on the basis of a calibration curve obtained by adding known amo...