Abstract
Urine of normal rats, pregnant animals and animals bearing chemically induced hepatoma was tested with antisera to foetoproteins by the double immunodiffusion technique. Antigens were not detected in the urine of normal rats. Alpha-foetoprotein was demonstrated in the urine of pregnant rats and hepatomabearing animals.
Highlights
THREE specific embryonic proteins have been described in the rat
Both foetoproteins are detected in the serum of rats with chemically induced hepatoma (StanislawskiBirencwajg, Uriel and Grabar,. 1967)
Since the alpha-foetoprotein (AFP) is present in the amniotic fluid, to which foetal urine contributes substantially (Pitkin, Reynolds and Burchell, 1968; Vernier and Smith, 1968), it is surprising that urinary excretion in the adult has received little attention (Masseyeff, 1972)
Summary
Animals.-Albino rats of the Hebrew University (Sabra) strain and randomly bred local rabbits, weighing 2-3 kg, were used. The absorbed antisera were tested by double immunodiffusion with the amniotic fluid and embryonic blood at a concentration of 10 mng of lyophilizate per ml of PBS. Group IV.-Eight female rats weighing 80 g at the beginning of the experiment were fed Miller's diet without 3'mDAB, and served as controls for the animals of Group III. Urine samples from rats of Group I and II were collected on 2 consecutive days. Urine samples from animals of Groups III and IV were collected once every 4 weeks as of the fourth month of the experiment. Unconcentrated urine specimens were tested with the specific antisera to amniotic fluid and embryonic blood by the double immunodiffusion technique. Blood was drawn from the tail vein on the day of urine collection and the serum was separated and tested with the 2 antisera. Sections were cut at 6 ,um and stained with haematoxylin and eosin
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
