Abstract
Two distinctly different preparations of the D{sub 1}-D{sub 2}-cytochrome b-559 photosystem II reaction center (RC) complex are shown to exhibit very similar 4.2 K absorption and hole-burned spectra in glasses. Absorption profiles for P680 and the active pheophytin are fully characterized with the mean zero-point energy of P680{sup *} lying {approx}25 cm{sup {minus}1} lower in energy than the Q{sub y} state of the latter. Decay of the accessory chlorophyll and pheophytin Q{sub y} states due to energy transfer is shown to be about 3 orders of magnitude slower than in bacterial RC.
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