Abstract
Excitation-emission matrix fluorescence (EEMF) coupled to chemometrics was used to explore essential oils (EOs). The spectrofluorometer was designed with basic and inexpensive materials and was accompanied by appropriate tools for data pre-treatment. Excitation wavelengths varied between 320nm and 600nm while emission wavelengths were from 340nm to 700nm. Excitation-emission matrix (EEM) spectra of EOs presented different features, revealing the presence of varying fluorophores. EOs from the same species but from different origins presented almost the same spectra, showing the possibility that EEM spectra could be used as additional parameters in the standardisation of EOs. With the aid of unfold principal component analysis (UPCA), resemblances obtained by spectral analysis of EOs were confirmed. A five components parallel factor analysis (PARAFAC) model was used to find the profiles of fluorophores in EOs. One of those components was associated to chlorophyll a.
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