Abstract
A new FRET pair has been reported based on the appearance of excimeric emission and hence significant spectral overlap between an otherwise non-FRET pair fluorophores viz. pyrene and rhodamine 6G in the anionic sodiumdodecylbenzenesulfonate (SDBS) micellar system. The occurrence of FRET based on such modulation of spectral overlap integral has not been dealt with in detail in comparison to the other FRET parameters like distance between the fluorophores and orientation. Due to the presence of benzene ring in the SDBS micellar system the formation of excimers is augmented due to the π-π interactions between the pyrene molecules and the benzene units of the surfactants. The excimeric pyrene has a bathochromically shifted peak which overlaps with the absorption peak of rhodamine 6G thereby giving rise to energy transfer as FRET between these otherwise non-FRET pair molecules. The efficiency of FRET between the pyrene and rhodamine 6G can be modulated by the degree of spectral overlap between the excimeric peak of pyrene and the absorption peak of rhodamime6g. All the experiments were carried in the Sodiumdodecylsulfate (SDS) micellar system also, which unlike SDBS does not possess any benzene unit, in order to compare the results of excimer formation as well the FRET process.
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