Abstract
Insoluble fatty surfaces are involved in many important interactions such as in biomembranes with soluble biological macro and micromolecules. In this paper we have studied the adsorption interaction of aqueous solution of DNA, some proteins and lactose on several sparingly soluble fatty substances namely milk fat, stearic acid, palmitic acid, phosphatidyl choline and cholesterol surfaces by measuring the depletion of the adsorbates by analytical methods. Adsorption () of DNA on the soft surfaces of stearic acid, milk fat, phosphatidyl choline, palmitic acid and cholesterol was measured as a function of DNA concentration C2. In each case was found to increase with C2 until it reached the maximum value at a critical concentration . For different surfaces stands in the order: stearic acid > milk fat > phosphatidyl choline > cholesterol > palmitic acid. DNA forms multilayers on stearic acid surface. Adsorption of hemoglobin on cholesterol surface is found to be negative or zero but that of BSA on cholesterol is positive. Adsorption of gelatin on cholesterol surface is significantly higher than that of BSA. Lysozyme on cholesterol surface forms multilayers and on casein forms bilayer. The lowering of free energies ?DGo for all systems have been calculated using integrated form of the Gibbs adsorption and their values have been compared with each other. It is concluded that despite differences in the adsorption behavior of the biomolecules on various soft surfaces, free energy change expressed as Bull’s free energy change (Δ) remain nearly constant except for BSA-fatty acid interaction which may be likely due a specific interaction.
Highlights
In this paper we have studied the adsorption interaction of aqueous solution of deoxyribonucleic acid (DNA), some proteins and lactose on several sparingly soluble fatty substances namely milk fat, stearic acid, palmitic acid, phosphatidyl choline and cholesterol surfaces by measuring the depletion of the adsorbates by analytical methods
Adsorption ( 12 ) of DNA on the soft surfaces of stearic acid, milk fat, phosphatidyl choline, palmitic acid and cholesterol was measured as a function of DNA concentration C2
Extents of adsorption of DNA on the surface of stearic acid, milk fat, and phosphatidylcholine have been carried out at pH 6.5 and ionic strength 0.10 maintained by NaCl (Figure 1(a))
Summary
Bull [1,2] in 1956-1957 first studied the adsorption of egg albumin and bovine serum albumin from solution onto the rigid surface of glass powder under various physico-chemical conditions. He measured specific surface areas covered due to packing of ellipsoid shaped egg-albumin and BSA molecules and analyzed the orientations of these globular proteins on the rigid glass surface. Extensive studies of relative adsorption of different soluble proteins in pure and mixed forms on different types of rigid surfaces of powdered substance of known specific surface areas have been studied by various workers [3,4,5,6,7,8]. Chattoraj and coworkers [16,17] have studied the excess adsorption of nucleic acids, BSA and gelatin from solution onto the surfaces of a few soft suspended particles like insoluble casein [13], cellulose [14], stearic acid particles [15]
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