Exceptionally well preserved late Quaternary plant and vertebrate fossils from a blue hole on Abaco, The Bahamas

Late Quaternary fossil deposits from The Bahamas, Cayman Islands, and Cuba contain fossils of the Cuban crocodile Crocodylus rhombifer. This species survives today only in Cuba and Isla de la Juventud (formerly Isla de Pinos); the populations in The Bahamas and Cayman Islands are locally extinct. Large fossil samples, including skulls, are known from underwater caves (blue holes) on Abaco in the northern Bahamas and organic peat deposits on Grand Cayman. Diagnostic cranial characters shared by the fossil crocodile skulls from Abaco and Grand Cayman and recent skulls of C. rhombifer from Cuba are: short, broad, and deep rostrum; prominent orbit; concave interorbital region and cranial roof; strong ridge on internal margin of the orbit and lateral margin of the cranial table; large, rounded protuberance on the posterolateral corner of the squamosal; premaxillary-maxillary suture on the palate transverse at the level of the first maxillary tooth; and 13 teeth in the maxilla. Using a ratio derived from living crocodylians of head length (premaxilla to parietal) to total length of about 1:7.2, approximate total lengths for fossil specimens of C. rhombifer from Abaco range from 1.3–2.3 m (mean 1.9 m) and Grand Cayman from 1.6–2.0 m (mean 1.7 m). Quaternary crocodylian remains are known from many other islands in The Bahamas, including Acklins, Crooked Island, Eleuthera, Grand Bahama, Mayaguana, New Providence, and San Salvador; however, most of these fossils are not complete enough for a species identification. Shells of an extinct species of the land tortoise Chelonoidis from Abaco and Mayaguana with crocodylian bite marks, jaws and teeth of capromyid rodents from Grand Cayman that appear to have been digested by a crocodylian, and data from carbon (13C/12C) isotopes derived from crocodile bones, as well as the lack of large freshwater vertebrates, suggest that C. rhombifer in The Bahamas and Cayman Island had a diet primarily consisting of terrestrial vertebrates. Evidence from stomach contents and behavioral ecology of extant Cuban crocodiles from Cuba further supports the terrestrial feeding habits of C. rhombifer. Radiocarbon (14C) dates on crocodile postcranial bones from Sawmill Sink and Dan’s Cave on Abaco range from 2,780–3,680 years Before Present (BP). Radiocarbon dates on peat associated with fossils of C. rhombifer from the Crocodile Canal site on Grand Cayman range from 375–860 years BP. These dates confirm that Cuban crocodiles survived into the late Holocene on Abaco and into the historic period on Grand Cayman. Evidence from a variety of sources, including radiocarbon dates, fossil and archaeological sites, and historical records confirms that the local extinction of crocodiles in The Bahamas and Cayman Islands occurred within the past 500 years, probably resulting from overhunting by humans. Fossils of C. rhombifer on Grand Cayman and Abaco, and probably throughout The Bahamas, verify that the Cuban crocodile was considerably more widespread in the West Indies during the Late Quaternary.

An extinct tortoise, Chelonoidis alburyorum n. sp., is described from nearly complete, beautifully preserved fossils from Sawmill Sink, a deep inland blue hole and cave system, on Great Abaco Island, Little Bahama Bank, in the northern Bahamas. This tortoise is part of an extensive fossil fauna in peat deposits associated with an immense debris cone in the entry shaft of this water-filled cave. The peat fauna also includes intact skeletons, skulls, and isolated bones of Cuban crocodiles (Crocodylus rhombifer), large birds, native rodents (Geocapromys ingrahami), and bats. The turtle remains include the first complete skull, first intact shells, and first associated vertebrae and appendicular skeletons of a tortoise from The Bahamas and/or West Indies. A morphological assessment of this tortoise and other Neotropical tortoises shows greater similarity of this new species with modern Chelonoidis nigra from the Galápagos Islands and fossils from the greater Caribbean area, than with the Cuban Chelonoidis cubensis or living and fossil continental Chelonoidis. AMS radiocarbon dates obtained from bones from the holotype and female paratype of the new tortoise, and from three Cuban crocodiles from Sawmill Sink, indicate a late Holocene age (2,580-3,820 yrs BP) for the peat deposits that produced the fossils of Chelonoidis alburyorum. Two other fossil faunas from this sink are thought to be older, possibly Pleistocene in age. These older fossils lack sufficient carbon to permit reliable radiometric dating. Chelonoidis alburyorum is one of a series of tortoise fossils recently found in blue holes, caves, and archaeological sites in the Bahamian archipelago, including the Turks and Caicos Islands, BWI. Two other fossil species from Abaco and the Turks and Caicos Islands, both in the genus Chelonoidis, are also new to science and will be described elsewhere. Affinities of the fossils from other Bahamian banks remain unstudied. A morphological asssessment of Neotropical tortoises shows greater similiarities between the new species, modern Cheolonoidis nigra, and to a lesser degree fossils from the greater Caribbean than with the Cuban Chelonoidis cubensis and continental Chelonoidis.

Late Pleistocene and Holocene vegetation changes and anthropogenic impacts in the Cauvery delta plains, southern India

Ancient DNA of extinct species from the Pleistocene and Holocene has provided valuable evolutionary insights. However, these are largely restricted to mammals and high latitudes because DNA preservation in warm climates is typically poor. In the tropics and subtropics, non-avian reptiles constitute a significant part of the fauna and little is known about the genetics of the many extinct reptiles from tropical islands. We have reconstructed the near-complete mitochondrial genome of an extinct giant tortoise from the Bahamas (Chelonoidis alburyorum) using an approximately 1 000-year-old humerus from a water-filled sinkhole (blue hole) on Great Abaco Island. Phylogenetic and molecular clock analyses place this extinct species as closely related to Galápagos (C. niger complex) and Chaco tortoises (C. chilensis), and provide evidence for repeated overseas dispersal in this tortoise group. The ancestors of extant Chelonoidis species arrived in South America from Africa only after the opening of the Atlantic Ocean and dispersed from there to the Caribbean and the Galápagos Islands. Our results also suggest that the anoxic, thermally buffered environment of blue holes may enhance DNA preservation, and thus are opening a window for better understanding evolution and population history of extinct tropical species, which would likely still exist without human impact.

A 2 m sediment core from Church's Blue Hole on Andros Island, Bahamas provides the first paleoecological record from the Bahama Archipelago. The timing of events in the lower portion of the core is uncertain due to inconsistencies in the radiocarbon chronology, but there is evidence that a late Holocene dry period altered the limnology of Church's Blue Hole and supported only dry shrubland around the site. The dry period on Andros may correlate with a widespread dry period in the Caribbean from 3200 to 1500 yr BP. After the dry period ended, a more mesic climate supported tropical hardwood thicket around Church's Blue Hole. At c. 740 radiocarbon yr BP there is a sudden rise in charcoal concentration and a rapid transition to pinewoods vegetation, while at c. 430 radiocarbon yr BP charcoal concentration drops, but is higher again near the top of the core. Although climatic shifts could have caused these changes in vegetation and charcoal concentration, the changes post-date human colonization of the Bahamas and may reflect human arrival, followed by the removal of humans c. 1530 AD and the recolonization of Andros Island c. 200 years later.

Ancient DNA from a 2,500-year-old Caribbean fossil places an extinct bird (Caracara creightoni) in a phylogenetic context

Late Quaternary fossils representing a locally extinct population of the Cuban crocodile (Crocodylus rhombifer) are reported from two underwater caves in the Dominican Republic. A large fossil sample of C. rhombifer, from Oleg's Bat Cave near Bavaro in the southeastern Dominican Republic, consists of four nearly complete skulls, numerous isolated cranial elements and mandibles, and more than 100 postcranial bones representing most of the skeleton. These fossils were collected from a completely submerged portion of the cave at a depth of 11 m and about 100 m from the nearest entrance. A skull, mandibles, and two vertebrae of a Cuban crocodile were also found in a second cave called Ni-Rahu, northeast of Santo Domingo.We identify the fossil crocodile skulls from the Dominican Republic as Crocodylus rhombifer because they share the following characters with modern skulls of C. rhombifer from Cuba (as well as fossil skulls from Cuba, the Bahamas, and Cayman Islands): short, broad, and deep rostrum; large orbits; convex nasals along the midline (midrostral boss); prominent swelling on the lacrimals anterior and medial to the orbits; low but obvious ridges extending anteriorly from the lacrimals to the nasals and posteriorly from the lacrimals to the prefrontals and frontals, outlining a distinct diamond- or rhomboid-shaped structure; strongly concave interorbital region and cranial roof; high, narrow ridges on the internal margins of the orbits, extending from the prefrontals to the frontals and posteriorly to the postorbitals; prominent ridges along the lateral margins of the cranial roof on the postorbitals and squamosals, terminating as noticeable protuberances on the posterolateral corners of the squamosals; premaxillary/maxillary suture on the palate essentially horizontal or transverse to the long axis of the skull at the level of the first maxillary tooth; 13 teeth in the maxilla.Certain aspects of the ecology and anatomy of living Crocodylus rhombifer in Cuba, and carbon isotope data from fossil crocodile bones from both the Dominican Republic and the Bahamas, indicate that the Cuban crocodile is a terrestrially adapted predator. The fossil deposits in Oleg's Bat Cave and other underwater caves in the Dominican Republic lack freshwater vertebrates, such as fish and turtles, but contain abundant samples of hystricognath rodents, small ground sloths, and other terrestrial vertebrates, including large land tortoises, that apparently were the primary prey of the crocodiles. Bats are abundant in the fossil deposits in Oleg's Bat Cave, and may have been an additional food source. Bone collagen from a tibia of C. rhombifer from Oleg's Bat Cave yielded an AMS radiocarbon date of 6460 ±30 ryrBP (equivalent to 7320 to 7430 cal yrBP). The chronology for the local extinction of C. rhombifer in Hispaniola is currently unknown, except to document the presence of this species in the eastern Dominican Republic in the early Holocene. Radiocarbon dates and historical records confirm that Cuban crocodiles survived into the period of European colonization (post-1492) in the Bahamas and on Grand Cayman. The only species of crocodile currently found in Hispaniola, the American crocodile (C. acutus), occurs in coastal marine habitats and in two inland brackishwater lakes: Lago Enriquillo in the Dominican Republic and the nearby Etang Saumâtre in Haiti. C. acutus has no fossil record in Hispaniola or elsewhere in the West Indies, suggesting that this species may be a very recent (late Holocene) immigrant in the Antillean region. Crocodylus rhombifer has one of the most limited geographic ranges of any living crocodylian species, known only from freshwater swamps in south-central Cuba and the Isla de Juventud (Isla de Pinos) off the southwestern coast of Cuba. Locally extinct or extirpated populations of C. rhombifer from fossil deposits in the Dominican Republic, Grand Cayman, and the Bahamas document a considerably wider distribution for this spe

To reconstruct the environmental history including vegetation, fire and climate dynamics, from the Cajanuma valley area ( 3285 m elevation) in the Podocarpus National Park, southern Ecuador , we address the following major research question: (1) How did the mountain vegetation developed during the late Glacial and Holocene? (2) Did fire played an important control on the vegetation change and was it natural or of anthropogenic origin?. Palaeoenvironmental changes were investigated using multiple proxies such as pollen, spores, charcoal analyses and radiocarbon dating. Pollen data indicated that during the late Glacial and transition to the early Holocene (ca. 16 000-10 500 cal yr BP) herb páramo was the main vegetation type around the study area, while subpáramo and mountain rainforest were scarcely represented. The early and mid-Holocene (ca. 10 500 to 5600 cal yr BP) is marked by high abundance of páramo during the early Holocene followed by a slight expansion of mountain forest during the mid-Holocene. During the mid- to late Holocene (ca. 5600-1200 cal yr BP) there is a significant presence of páramo and subpáramo while Lower Mountain Forest decreased substantially, although, Upper Mountain Forest remained relatively stable during this period. The late Holocene, from ca. 1200 cal yr BP to present, was characterized by páramo; however, mountain forest and subpáramo presented significantly abundance compared to the previous periods. Fires became frequent since the late Holocene. The marked increased local and regional fire intensity during the wetter late Holocene strongly suggests that were of anthropogenic origin. During the late Glacial and early Holocene, the upper forest line was located at low elevations; but shifted slightly upslope to higher elevations during the mid-Holocene.

Environmental conditions and the emergence of ceramics in the Late Pleistocene-Early Holocene at the Krasnaya Gorka site in the Transbaikal region, Southern Siberia

Our understanding of glacial isostatic rebound across Patagonia is highly limited, despite its importance to constrain past ice volume estimates and better comprehend relative sea-level variations. With this in mind, our research objective is to reconstruct the magnitude and rate of Late Glacial and Holocene glacial isostatic adjustment near the center of the former Patagonian Ice Sheet. We focus on Larenas Bay (48°S; 1.26 km2), which is connected to Baker Channel through a shallow (7.4 m) and narrow (ca. 150 m across) inlet, and hence has the potential to record periods of basin isolation and marine ingression. The paleoenvironmental evolution of the bay was investigated through a sedimentological analysis of a 9.2 m long radiocarbon-dated sediment core covering the last 16.8 kyr. Salinity indicators, including diatom paleoecology, alkenone concentrations and CaCO3 content, were used to reconstruct the bay's connectivity to the fjord. Results indicate that Larenas Bay was a marine environment before 16.5 cal kyr BP and after 9.1 cal kyr BP, but that it was disconnected from Baker Channel in-between. We infer that the postglacial rebound started before 16.5 cal kyr BP and that it outpaced global sea-level rise until slightly before 9.1 cal kyr BP. During the Late Glacial and early Holocene, the center of the former Patagonian Ice Sheet experienced an absolute uplift of ca. 96 m, at an average rate of 1.3 cm/yr. During the remainder of the Holocene, glacial isostatic adjustment continued (ca. 20 m), but at a slower average pace of 0.2 cm/yr. Comparisons between multi-millennial variations in the salinity indicators and existing records of global sea-level rise suggest that the glacial isostatic adjustment rate also fluctuated within these time intervals, likely in response to glacier dynamics. More specifically, most of the glacial isostatic adjustment registered between 16.5 – 9.1 cal kyr BP seems to have occurred before meltwater pulse 1A (14.5 – 14.0 kyr BP). Likewise, it appears that the highest glacial isostatic rebound rates of the last 9.1 kyr occurred during the late Holocene, most likely in response to glacier recession from their Neoglacial maxima. This implies a relatively rapid response of the local solid earth to ice unloading, which agrees with independent modelling studies investigating contemporary uplift. We conclude that the center of the former Patagonian Ice Sheet experienced a glacial isostatic adjustment of ca. 116 m over the last 16.5 kyr, and that >80% occurred during the Late Glacial and early Holocene.

In order to study the stability and dynamics of highly biodiverse mountain rain forest and paramo ecosystems, the late Pleistocene and Holocene climate and fire variability as well as human impact in the Podocarpus National Park region, the first comprehensive palaeoenviremental study from the southeastern Ecuadorian Andes will be presented. An initial study of recent vegetation/modern pollen rain relationship on an altitudinal transects between 1800 and 3200 m elevation in the mountain forest and (sub)-paramo vegetation of the ECSF (Estacion Cientifica San Francisco) research area provide important background information in the interpretation of late Quaternary pollen records. Cluster analysis on the pollen rain data by CONISS, clearly reflects the zonation of the different vegetation units, lower mountain forest (LMF), upper mountain forest (UMF) and (sub)-paramo. A relatively high number of pollen taxa correspond to the altitudinal distribution of genera and families of modern vegetation. The modern tree line in the research area is at ca. 2790 m, which is pointed out by an increase of (sub)-paramo taxa and a decrease of mountain forest taxa in the pollen rain data. Palaeoenvironmental changes, inferred from 9 lake, peat bog and soil deposits, collected at different elevations between ca. 2000 and 3300 m, were investigated by pollen, spores and charcoal analysis, in combination with XRF- and magnetic susceptibility-scanning on the lake sediment cores. During the late Pleistocene from ca. 21,000 to 11,200 cal yr BP, grass-paramo vegetation occurred at the Tiro-Pass (2810 m), reflecting cold and moist climatic conditions as well as a shift of vegetation zones into lower elevation during this period. During the transition from late Pleistocene to early Holocene from ca. 14,500 to 9700 cal yr BP, arboreal taxa, mainly Weinmannia strongly increase at Laguna Cocha Caranga (2710 m), reflecting a raise in temperature. The early Holocene from ca. 11,200 to 8900 cal yr BP (El Tiro-Pass) is characterised by an increase of temperature and moisture, as well as a shift of vegetation zones into higher elevation. During the mid Holocene period, from ca. 8900 to 3300 cal yr BP, upper mountain forest vegetation developed at the El Tiro-Pass, where subparamo vegetation occurred in recent times, suggesting a warmer climate than present day at this elevation. XRF-scanning data from Laguna Rabadilla de Vaca (3310 m) reflects a drier period from ca. 8990 to 6380 cal yr BP and a wetter period from ca. 6380 to 3680 cal yr BP. The green algae Botryococcus braunii, Isoetes and Cyperaceae were used to reconstruct Holocene wet/dry phases at Laguna Cocha Caranga. Drier climatic conditions occurred from ca. 9700 to 6900 cal yr BP and from ca. 4200 to 1300 cal yr BP. From ca. 6900 to 4200 cal yr BP and from ca. 1300 cal yr BP to modern time"s wetter climatic conditions occurred. During the late Holocene, modern climatic conditions, as well as recent vegetation established since ca. 3680 cal yr BP at Laguna Rabadilla de Vaca and since ca. 3300 cal yr BP at the El Tiro-Pass. An increase of fire intensity during the early to mid Holocene period after ca. 9700 cal yr BP at Laguna Cocha Caranga and after about 7500 cal yr BP at the El Tiro-Pass reflects beginning human impact on the ecosystem in the Podocarpus National Park region. High occurrence of grasses document, that past fires have markedly influenced the floristic composition of the mountain rain forest and paramo ecosystems during the mid to late Holocene period. The reduction of fire intensity coupled with a decrease of grasses after ca. 1300 cal yr BP (Laguna Cocha Caranga) and between ca. 970 to 400 cal yr BP (Upper Rio San Francisco valley), coupled with a missing of Zea mais pollen, suggests a reduction and/or absence of human activities, may be as a result of political unrest. After the reduction and/or absence of human influence the mountain forest vegetation starts to recover.

Seismic stratigraphy of the Blue Hole (Lighthouse Reef, Belize), a late Holocene climate and storm archive

Timing of major forest compositional changes and tree expansions in the Retezat Mts during the last 16,000 years

Dissolution of carbonate platforms, like The Bahamas, throughout Quaternary sea-level oscillations have created mature karst landscapes that can include sinkholes and off-shore blue holes. These karst features are flooded by saline oceanic waters and meteoric-influenced groundwaters, which creates unique groundwater environments and ecosystems. Little is known about the modern benthic meiofauna, like foraminifera, in these environments or how internal hydrographic characteristics of salinity, dissolved oxygen, or pH may influence benthic habitat viability. Here we compare the total benthic foraminiferal distributions in sediment-water interface samples collected from <2 m water depth on the carbonate tidal flats, and the two subtidal blue holes Freshwater River Blue Hole and Meredith’s Blue Hole, on the leeward margin of Great Abaco Island, The Bahamas. All samples are dominated by miliolid foraminifera (i.e., Quinqueloculina and Triloculina), yet notable differences emerge in the secondary taxa between these two environments that allows identification of two assemblages: a Carbonate Tidal Flats Assemblage (CTFA) vs. a Blue Hole Assemblage (BHA). The CTFA includes abundant common shallow-water lagoon foraminifera (e.g., Peneroplis, Rosalina, Rotorbis), while the BHA has higher proportions of foraminifera that are known to tolerate stressful environmental conditions of brackish and dysoxic waters elsewhere (e.g., Pseudoeponides, Cribroelphidium, Ammonia). We also observe how the hydrographic differences between subtidal blue holes can promote different benthic habitats for foraminifera, and this is observed through differences in both agglutinated and hyaline fauna. The unique hydrographic conditions in subtidal blue holes make them great laboratories for assessing the response of benthic foraminiferal communities to extreme environmental conditions (e.g., low pH, dysoxia).

Dissolution of carbonate platforms, like The Bahamas, throughout Quaternary sea-level oscillations have created mature karst landscapes that can include sinkholes and off-shore blue holes. These karst features are flooded by saline oceanic waters and meteoric-influenced groundwaters, which creates unique groundwater environments and ecosystems. Little is known about the modern benthic meiofauna, like foraminifera, in these environments or how internal hydrographic characteristics of salinity, dissolved oxygen, or pH may influence benthic habitat viability. Here we compare the total benthic foraminiferal distributions in sediment-water interface samples collected from < 2 m water depth on the carbonate tidal flats, and the two subtidal blue holes Freshwater River Blue Hole and Meredith’s Blue Hole, on the leeward margin of Great Abaco Island, The Bahamas. All samples are dominated by miliolid foraminifera (i.e., Quinqueloculina and Triloculina), yet notable differences emerge in the secondary taxa between these two environments that allows identification of two assemblages: a Carbonate Tidal Flats Assemblage (CTFA) vs. a Blue Hole Assemblage (BHA). The CTFA includes abundant common shallow-water lagoon foraminifera (e.g., Peneroplis, Rosalina, Rotorbis), while the BHA has higher proportions of foraminifera that are elsewhere known to tolerate stressful environmental conditions of brackish and dysoxic waters elsewhere (e.g., Pseudoeponides, Cribroelphidium, Ammonia). We also observe how the hydrographic differences between subtidal blue holes can promote different benthic habitats for foraminifera, and this is observed through differences in both agglutinated and hyaline fauna. The unique hydrographic conditions in subtidal blue holes make them great laboratories for assessing the response of benthic foraminiferal communities to extreme environmental conditions (e.g., low pH, dysoxia).