Examination the effects of chestnut and Manuka Honey for wound healing on mice experimental model

Abstract

Objective: In this study, the aim is to examine the effects of Chestnut and Manuka honey on wound healing in order to investigate the effectiveness of cost-effective alternative to current approaches in terms of wound care and treatment. Material and Methods: In this study, we used 30 healthy male Balbc mice weighing 18-24 g. We randomly divided the rats into three groups. A control group, a group treated with only Chestnut honey, a group treated with Manuka honey cream. After the wounds were formed in groups, the tissue samples were gathered on the seventh and fourteenth days. Then these samples were examined histologically and immunohistochemically. Results: When the study results were evaluated, statistically significant differences were seen between histological and immune-histochemical findings in wound tissue preparations. On the seventh day, tissue samples showed re-epithelialization (P=0,002), granulation cell density (P=0,003) and angiogenesis (P= 0,003). In the fourteenth day tissue samples, we found epithelialization (P=0,001), granulation cell density (P=0,002) and angiogenesis (P=0,001). In the tissue samples in the seventh and fourteenth days between the groups, we found immino-histochemically, Ki-67 and EGF dyeing percentages as P= 0,004 and P=0,003 respectively. Conclusion: We think chestnut honey may contribute to a shorter wound healing process.