Examination of the Ovarian Reserve after Generation of Unilateral Rudimentary Uterine Horns in Rats

Hasan Toyganözü,Murat Api,Hakan Aytan,Deniz Satar,Sinem Büyüknacar,Raziye Narin,Hakan Nazik,Mehmet Ali Narin

doi:10.1155/2014/918496

Hasan Toyganözü, Murat Api + Show 6 more

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https://doi.org/10.1155/2014/918496

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Abstract

Objective. The purpose of this experimental rat model study is to evaluate the changes in the ovarian environment after excision of the rudimentary horn. Methods. Ten female Wistar albino rats were used in this study. One cm of right uterine horn length was excised in the first operation. Two months after the first operation, all animals were sacrificed to obtain ovaries for histological examination. Mann-Whitney U test and Student's t-test were used for statistical analysis purposes. Statistical significance was defined as P < 0.005. Results. The number of primordial follicles (P = 0.415), primary follicles (P = 0.959), preantral follicles (P = 0.645), antral follicles (P = 0.328), and Graafian follicles (P = 0.721) was decreased and the number of atretic follicles (P = 0.374) increased in the right ovarian side. Howeve,r this difference was not found to be statistically significant. Conclusion. The results of this experimental rat model study suggest that the excision of rudimentary horn could have negative effects on ipsilateral ovarian functions.

Highlights

Congenital Mullerian anomalies account for the majority of uterus anomalies
The classification of the American Society for Reproductive Medicine (ASRM) divides this anomaly group into four subgroups: cavity rudimentary horn communicating with the unicornuate uterus, noncommunicating cavity rudimentary horn, no-cavity rudimentary horn, and unicornuate uterus without rudimentary horn
It has been previously shown that tubal ligation and hysterectomy affected the blood flow to the ovary and decreased the ovarian reserve [5,6,7,8,9,10,11,12,13]

Summary

Introduction

Congenital Mullerian anomalies account for the majority of uterus anomalies. The prevalence of uterine anomaly in the general population is estimated to be 0.5% [1]. Unicornuate uterus occurs in the event of arrest or failure of development of one Mullerian duct. The incidence of unicornuate uterus in uterine anomalies has been reported at 14% [2]. A unicornuate uterus may lead to many gynecological or obstetric complications. They include infertility, dysmenorrhea, endometriosis, and premature birth [3, 4]. If pregnancy occurs in the rudimentary horn, there is high risk of uterine rupture. It is suggested that the rudimentary horn is excised as soon as it is diagnosed due to such risks [4]. No sufficient data is available covering the effects of the excision of a rudimentary uterine horn that is on the ovarian reserve located on the same side. It has been previously shown that tubal ligation and hysterectomy affected the blood flow to the ovary and decreased the ovarian reserve [5,6,7,8,9,10,11,12,13]

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Results

Conclusion