Abstract
Laboratory studies of bacterial gene expression have not allowed the investigation of in vivo factors crucial to pathogenesis. Recently, genetic tools have been used to identify in vivo-activated genes. These tools, however, have not allowed the measurement of specific expression levels of virulence-associated bacterial genes in vivo. This report describes a system, previously tested utilizing the fructosyltransferase (ftf) gene of Streptococcus mutans, that allows the measurement of activity of specific virulence-associated streptococcal genes while bacteria are living in the animal environment.
Published Version
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