Examination of some properties of dermatophytes for long-term preservation using periodic transfer method.

Abstract

ABSTRACTSeventy‐two strains from species of dermatophytes which had been transferred periodically on Sabouraud's dextrose agar for more than ten years were examined for their morphology, physiological activities and biological properties.Of 42 strains of Trichophyton rubrum, 17 (40.4%) were positive for pigment production on Sabouraud's dextrose agar. In mating experiments, 15 (79%) of 19 strains tested were compatible with Arthroderma simii and proved to be of the “—” mating type. Eight (67%) of 12 strains in the Microsporum gypseum complex were positive for macroconidium formation. In mating experiments, 4 of 12 strains proved to be Nannizzia gypsea of the “+” type, 3 to be of the “—” type, and one to be N. incurvata of the “—” type.Most of the 18 strains in the T. mentagrophytes complex and M. canis have been maintained with alterations.