Abstract
Amniotic membrane as a cell culture scaffold is a useful membrane in regenerative medicine. We established a method to create a cultured oral mucosal epithelial sheet using the amniotic membrane as a scaffold, and applied this sheet clinically. Satisfactory results were obtained without defects such as rejection. The amniotic membrane has been demonstrated to be a suitable scaffold for cell culture, and has provided novel, useful and effective therapies in regenerative medicine. Additionally, this cell culture system was applied to the culture of periodontal membrane-derived cells. Periodontal ligament-derived cells proliferated on the amniotic membrane, and showed desmosomes and intercellular junctions, such as tight junctions, and thus successfully created a cultured periodontal ligament sheet (ref. 1, 2).
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