Examination of bacteria content of leachate concentration in open dumpsites, at Gwagwalada, Federal Capital Territory, Abuja, Nigeria

Abstract

The bacteria content of leachate concentration in open dumpsite is a threat to man and its environment if not properly managed. One of the negative impacts of bacteria in leachate is that it may pollute water system which may lead to diseases if not prevented. In this study, the leachate concentrates were collected from five different location of open dumpsite in Gwagwalada town and examined bacteriologically. Using pour plate method, bacteria were counted and isolated. Biochemical tests and Molecular identification of bacteria isolate based on DNA extraction, DNA quantification and 16S rRNA Amplification were carried out. The total coliform bacterial counts ranged from 4.1× 105cfu/ml - 4.8×105cfu/ml. Isolated bacteria with percentage occurrence were Staphylococcus spp. (37.14%), Klebsiella aerogenes (25.71%), Proteus spp. (20%), Priestia megaterium (11.43%), Pseudomonas stutzeri (2.86%) and Enterobacter hormaechei (2.86%). Generally, both gram positive organisms showed 100% susceptibility to Pefloxacin and Cotrimoxazole and exhibited 100% resistance to Ampliclox while all gram negative bacteria showed 100% susceptibility to Ciprofloxacin. The biochemical test and molecular identification of bacteria isolates showed the presence of pathogenic bacteria which is hazardous to human health within the environment due to the possibility of contamination of drinkable water. As a result, the government and environmental agency should work together in preventing the situation of open dumpsite around residential areas through educational and awareness program.