Abstract
Elderberry (Sambucus nigra subsp. nigra) is a very popular crop in Europe and its importance is increasing as a distinguished source of natural food colorants. The amount of natural pigments in fruits depends on several factors such as the plant cultivar, the geographical origin, the climatic conditions, and the time of harvest. Cyanidin-3-O-sambubioside-5-O-glucoside, cyanidin-3-O-sambubioside and cyanidin-3-O-glucoside were identified in elderberry fruit by liquid chromatography-mass spectrometry (LC-MS), and quantified by high performance liquid chromatography (HPLC-Vis). In a fruit maturity study, âSamoccoâ had the highest water soluble solid content compared with two other elderberry cultivars grown at two locations. Anthocyanin profile and content also differed by cultivar. With âSamoccoâ, cyanidin-3-O-sambubioside accounted for more than 50% of all analysed anthocyanins, but the amount of this pigment was less in the other cultivars. âSamoccoâ had higher levels of total anthocyanins compared with most other cultivars at the two locations. Anthocyanin content was influenced by change of pH value and by heat treatment. After heat treatment, the highest anthocyanin concentration was observed at pH 3.5 with âHaschbergâ, and at pH 3.0 with âSamoccoâ. Total anthocyanin content was decreased 7% by heat treatment with âHaschbergâ and by 25% with âSamoccoâ at the original pH value of 4.0.
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