Examination methicillin-resistant Staphylococcus aureus (MRSA) prevalence in cockroaches from hospital in Chaharmahal-va-Bakhtiari province, Iran by polymerase chain reaction (PCR)

Abstract

This study gives the reported prevalence of cockroaches and the medical importance of theinsects due to the transmission of nosocomial infections. Besides, one of the major reasons of hospital and community infections all over the world is methicillin-resistant Staphylococcus aureus(MRSA). The aim of this study was to determine inset the hospital cockroaches as the main factor for MRSA infections transmission and also determine antibiogram pattern MRSA. In this study, going to hospitals, over 100 cockroaches were collected using hand and Telesticky methods and enriching the intended strain on a specific medium. Then was a designed primer for mecA gene and amplification in polymerase chain reaction (PCR). The disk diffusion method was used for specifying resistance patterns in line with guidelines of Clinical and Laboratory Standards Institute(CLSI). Results showed that a total of 44 (62.86%) of 100 samples were contaminated with S. aureus isolated from cockroaches hospitals, also with molecular sieve of PCR, an addition of 8 (19.56%) of the strain contained the mecA gene. The overall resistance of isolated MRSA strains to antimicrobial agents was 8 (100%) for methicillin, 7 (87.5%) for cefixime and 6 (75%) for vancomycin, which had more resistance, respectively. This study implies that cockroaches, as a potential factor in transmission of MRSA function and medical resistance pattern of MRSA are different in different areas. Key words: Antibiogram, resistance, methicillin-resistant Staphylococcus aureus (MRSA), Chaharmahal-va-Bakhtiari hospitals, polymerase chain reaction (PCR).