Exaggerated stress-induced release of nonesterified fatty acids in JCR:LA-corpulent rats

Abstract

Studies were performed to test the hypothesis that the stress response is exaggerated in obesity and to identify which component of the response is modified. Chronically cannulated lean (+/+) and obese ( cp cp ) JCR:LA rats were subjected to mild restraint stress for 15 minutes. Blood pressure and serum glucose, insulin, and corticosterone responses did not differ significantly between genotypes before, during, or after restraint. Lean rats had a significantly greater plasma epinephrine (EPI) response but a similar norepinephrine (NE) response compared with obese rats. Serum nonesterified fatty acid (NEFA) concentrations were unchanged in lean rats, but increased from 0.86 to a mean of 1.48 mmol/L in obese rats within 10 minutes of restraint. All animals recovered to prestress values by 45 minutes postrestraint. In obese rats, handling increased NEFAs to greater than 2 mmol/L before or at 165 minutes after restraint. In lean rats, NEFAs increased when handling occurred at 165 minutes after restraint, but there was no significant NEFA response at the prerestraint point. The sensitivity of adipose tissue to NE-induced lipolysis was not significantly different between genotypes. It is concluded that the exaggerated accumulation of NEFAs in the blood of obese rats results from increased adipose tissue mass. These increases in NEFAs in obese rats resulting from mild stress reached levels normally associated with gross pathology such as ketotic diabetes.