Exaggerated Autophagy in Stanford Type A Aortic Dissection: A Transcriptome Pilot Analysis of Human Ascending Aortic Tissues.

Zeyi Zhou,Qing Zhou,Jie Du,Yali Wang,Junxia Wang,Xiyu Zhu,Xinlong Tang,Yan Liu,Dongjin Wang,Can Xu

doi:10.3390/genes11101187

Abstract

Stanford type A aortic dissection (TAAD) is one of the most dangerous diseases of acute aortic syndrome. Molecular pathological studies on TAAD can aid in understanding the disease comprehensively and can provide insights into new diagnostic markers and potential therapeutic targets. In this study, we defined the molecular pathology of TAAD by performing transcriptome sequencing of human ascending aortic tissues. Pathway analysis revealed that activated inflammation, cell death and smooth muscle cell degeneration are the main pathological changes in aortic dissection. However, autophagy is considered to be one of the most important biological processes, regulating inflammatory reactions and degenerative changes. Therefore, we focused on the pathological role of autophagy in aortic dissection and identified 10 autophagy-regulated hub genes, which are all upregulated in TAAD. These results indicate that exaggerated autophagy participates in the pathological process of aortic dissection and may provide new insight for further basic research on TAAD.

Highlights

Acute aortic dissection is a sudden onset of blood leaving the normal aortic lumen through an intimal tear and rapidly dissecting the inner layers from the outer layers of the media to produce a false lumen [1,2]
To detect whether autophagy contributes to the development of type A aortic dissection (TAAD) [12], we identified autophagy-related genes (ARGs) from the Autophagy
We measured the inner diameter of the ascending aorta, where the largest dilation occurred, and we considered this as the aortic diameter, based on the results of the preoperative computed tomography angiography (CTA) examination

Summary

Introduction

Acute aortic dissection is a sudden onset of blood leaving the normal aortic lumen through an intimal tear and rapidly dissecting the inner layers from the outer layers of the media to produce a false lumen [1,2]. Stanford type A aortic dissection, known as type A aortic dissection (TAAD), is one of the most common dissections [3]. With the continuous advancement of gene sequencing technology, molecular pathology has gradually become an independent discipline of science. This technology provides better diagnostic indicators and facilitates the development of treatment methods for many diseases [5,6], especially cancers. Previous studies on aortic dissection have always used animal models, which cannot aid in fully understanding its pathogenesis owing to various limitations. We used human ascending aortic tissues for our study. We performed whole transcriptome sequencing to acquire differential gene expression profiles in 10 TAAD tissues and 10 normal tissues

Methods

Results

Conclusion