Abstract
Colorectal cancer is one of the most common and often fatal cancers in humans, but it has the highest chance of a cure if detected at an early precancerous stage. Carcinogenesis in the colon begins as an uncontrolled growth forming polyps. Some of these polyps can finally be converted to colon cancer. Early diagnosis of adenomatous polyps is the main approach for screening and preventing colorectal cancer, and vibration spectroscopy can be used for this purpose. This work is focused on evaluating FTIR and Raman spectroscopy as a tool in the ex vivo analysis of colorectal polyps, which could be important for the early diagnosis of colorectal carcinoma. Multivariate analyses (PCA and LDA) were used to assist the spectroscopic discrimination of normal colon tissue, as well as benign and malignant colon polyps. The spectra demonstrated evident differences in the characteristic bands of the main tissue constituents, i.e., proteins, nucleic acids, lipids, polysaccharides, etc. Suitable models for discriminating the three mentioned diagnostic groups were proposed based on multivariate analyses of the spectroscopic data. LDA classification was especially successful in the case of a combined set of 55 variables from the FTIR, FT Raman and dispersion Raman spectra. This model can be proposed for ex vivo colorectal cancer diagnostics in combination with the colonoscopic extraction of colon polyps for further testing. This pilot study is a precursor for the further evaluation of the diagnostic potential for the simultaneous in vivo application of colonoscopic Raman probes.
Highlights
4th Internal Clinic—Gastroenterology and Hepatology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 00 Prague, Czech Republic; Abstract: Colorectal cancer is one of the most common and often fatal cancers in humans, but it has the highest chance of a cure if detected at an early precancerous stage
The aim of this work is an evaluation of vibrational spectroscopic methods, i.e., Fourier transform (FT) infrared spectroscopy (FTIR) with attenuated total reflection (ATR), FT Raman, and dispersion Raman spectroscopy as tools in the ex vivo analysis of colorectal polyps for the identification stage development of adenomatous polyps, which might be important for the early diagnosis of colorectal carcinoma
Average vibrational spectra of control colon tissues (n = 10), adenomatous (n = 7) and adenocarcinomatous (n = 3) colon polyps and the corresponding difference spectra are represented in Figure 1 left and right panels, respectively; the FTIR (900–3750 cm−1 ), FT Raman (500–3100 cm−1 ) and dispersion Raman (400–3100 cm−1 ) spectra are grouped in the top, middle and bottom panels, respectively
Summary
4th Internal Clinic—Gastroenterology and Hepatology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, U Nemocnice 2, 128 00 Prague, Czech Republic; Abstract: Colorectal cancer is one of the most common and often fatal cancers in humans, but it has the highest chance of a cure if detected at an early precancerous stage. LDA classification was especially successful in the case of a combined set of 55 variables from the FTIR, FT Raman and dispersion Raman spectra This model can be proposed for ex vivo colorectal cancer diagnostics in combination with the colonoscopic extraction of colon polyps for further testing. Carcinogenesis in the colon or rectum begins as uncontrolled growth in the inner layers towards the intestinal lumen, forming polyps Some of these polyps, known as neoplastic or adenomatous, can undergo a gradual transformation, and most of them can be converted to colon cancer [2,3]. Colonoscopic screenings have significantly increased the survival rate of patients with colorectal cancer, it remains a challenge to distinguish adenomas and early adenocarcinomas from benign hyperplastic polyps using colonoscopy. This difficulty is mainly because conventional white light reflection colonoscopy relies heavily on the subjective visual assessment of colorectal polyps
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
