Ex Vivo Glucose Detection in Human Blood Serums with Carbon Quantum Dot-Doped Oleic Acid-Treated Chitosan Nanocomposites.

Abstract

Herein, carbon quantum dot (CQD)-doped oleic acid (OL)-treated chitosan (Ch) nanocomposites (Ch-OL/CQD) are prepared by a simple solution technique for nonenzymatic ex vivo detection of glucose in human blood samples. From the architecture of the structure, it is observed that the agglomeration of CQD is restricted by OL-treated Ch polymeric chain, and simultaneously the inhibition in the entanglement of Ch-OL polymeric chains in the matrix is attained by the incorporation of CQD, thereby proving the high stability of the nanocomposite. In vitro detection of glucose is studied by the "Turn ON-OFF" fluorescence technique which is again evidenced by the shining core image of nanocomposites in HRTEM. A highly selective glucose sensing against interfering sugars due to the specific spatial arrangement of the hydroxyl groups of glucose, leading to prominent hydrogen-bonding interaction is established, with a very low limit of detection (LOD) of 1.51 μM, covering a wide linear domain from 0 to 104 μM, R2 = 0.98. Moreover, the calculated glucose levels in real human blood serums by Ch-OL/CQD nanocomposites are compared with a commercial glucometer, with recovery percentages from 95.8 to 107.3%. The clinical potential is supported by studying the stimuli responsiveness of the nanocomposites as a function of pH and ionic strength, encouraging the operation of the sensor in a complex biological scenario. The present work may offer an opportunity for the monitoring of glucose in the blood for successful diabetes management.