Ex-situ conservation of the native orchid Coelogyne rochusseni de Vriese from the Bukit Rimbang and Bukit Baling Wildlife Reserve Areas

Pebra Heriansyah,Gusti Marlina

doi:10.24252/bio.v9i1.21274

Abstract

The native orchid Coelogyne rochussenii de Vriese is under threat of extinction, so conservation measures need to be taken. In-situ conservation is constrained by time, resources and costs, and the appropriate conservation must be ex-situ. Therefore, This study aims to determine the most effective method for ex-situ conservation, especially basal medium and activated charcoal, in order to determine the fastest germination. The method used in this research was a factorial completely randomized design, consisting of four different basal medium treatments, Knudson C, Vacin and Went, Murashige and Skoog, and Hyponex + Vitamin medium. This treatment was combined with the application of activated charcoal at 4 different levels, with concentrations of 0.1,2.3 g/L-1, the parameters observed were germination day (day), germination percentage (%), and percentage of contamination (%). The results of this study concluded that the conservation of the Native orchid Coelogyne Rochussenii de Vriese using various growing mediums and activated charcoal had a significant effect on the growth of the embryo culture native orchid. Hyponex + Vitamin medium with 3 g/L-1 is able to accelerate germination days to 29.33 days, increase germination percentage to 92.06%, and reduce contamination to 0%.

Highlights

The orchid Coelogyne rochussenii de Vriese is a widely distributed plant across Southern Thailand, Peninsular Malaysia, Singapore, Kalimantan, Sumatra, and Java Island, Sulawesi, Maluku, and Palawan in the Philippines (Lok et al, 2011).Native orchids are threatened with extinction both locally and globally, this condition is triggered by the plundering of the plants for commercial purposes (Ticktin et al, 2020)
The fastest germination day (29.33) was found in the Hyponex + vitamin medium combined with 3 g/L-1 activated charcoal media
The combination of activated charcoal with Knudson C (KC), Vacin & Went (VW), and Murashige & Skoog (MS) media, showed that the 3 g/L-1 activated charcoal concentration produced the fastest germination compared to the control (Fig. 1)

Summary

Introduction

The orchid Coelogyne rochussenii de Vriese is a widely distributed plant across Southern Thailand, Peninsular Malaysia, Singapore, Kalimantan, Sumatra, and Java Island, Sulawesi, Maluku, and Palawan in the Philippines (Lok et al, 2011).Native orchids are threatened with extinction both locally and globally, this condition is triggered by the plundering of the plants for commercial purposes (Ticktin et al, 2020). The orchid Coelogyne rochussenii de Vriese is a widely distributed plant across Southern Thailand, Peninsular Malaysia, Singapore, Kalimantan, Sumatra, and Java Island, Sulawesi, Maluku, and Palawan in the Philippines (Lok et al, 2011). Native orchid extinctions are caused by the dependence of the plant on mycorrhizal fungi and insect pollinators which are disturbed by the imbalance in the ecosystem (Kolanowska et al, 2020). The habitat of this plant has been eroded by the increasingly disrupted forest conditions. There is degradation of the buffer areas in the Bukit Rimbang and Baling Wildlife Reserve areas up to 82.25% (Suandy et al, 2014), conservative measures are needed to prevent orchids from extinction

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Results