Abstract
Protein disulphide isomerase (PDI) is an oxidoreductase enzyme abundant in the endoplasmic reticulum (ER). In plants, PDIs have been shown to assist the folding and deposition of seed storage proteins during the biogenesis of protein bodies in the endosperm. Cloning and characterization of the complete set of genes encoding PDI and PDI like proteins in bread wheat (Triticum aestivum cv. Chinese Spring) and the comparison of their sequence, structure and expression with homologous genes from other plant species were reported in our previous publications. Promoter sequences of three homoeologous genes encoding typical PDI, located on chromosome group 4 of bread wheat, and PDI promoter sequence analysis of Triticum urartu, Aegilops speltoides and Aegilops tauschii had also been reported previously. In this study, we report the isolation and sequencing of a ~700 bp region, comprising ~600 bp of the putative promoter region and 88 bp of the first exon of the typical PDI gene, in five accessions each from Triticum urartu (AA), Aegilops speltoides (BB) and Aegilops tauschii (DD). Sequence analysis indicated large variation among sequences belonging to the different genomes, while close similarity was found within each species and with the corresponding homoeologous PDI sequences of Triticum aestivum cv. CS (AABBDD) resulting in an overall high conservation of the sequence conferring endosperm-specific expression.
Highlights
Wheat is adapted to temperate regions of the world and was one of the first crops to be domesticated
We report the isolation and sequencing of a ~700 bp region, comprising ~600 bp of the putative promoter region and 88 bp of the first exon of the typical Protein disulphide isomerase (PDI) gene, in five accessions each from Triticum urartu (AA), Aegilops speltoides (BB) and Aegilops tauschii (DD)
PDI promoter sequence analysis of Triticum urartu, Aegilops speltoides and Aegilops tauschii was given by [32].This paper reports variability in a ~700 bp region, comprising ~600 bp of the 5’ upstream putative promoter region and 88 bp of the first exon of the typical PDI gene from the diploid species Triticum urartu (AA) Aegilops speltoides (BB) and Aegilops tauschii (DD)
Summary
Wheat is adapted to temperate regions of the world and was one of the first crops to be domesticated. The diploid progenitors of the three genomes A, B, and D have been identified in Triticum urartu, Aegilops speltoides and Aegilops tauschii, the progenitor of B genome is still matter of debate [1]. Throughout their evolutionary history, multiple polyploidization events occurred between species of the Triticum and Aegilops genera and human manipulation of wild species led to the domestication of different cultivated lineages [2,3,4,5]. The second polyploidisation event occurred between the new tetraploid species (AABB) and diploid Aegilops tauschii (DD genome) to give rise to hexaploid wheat (AABBDD) Triticum aestivum [8,11]. A recent study revealed lower levels of polymorphism in the D genome than in the A and B genome, indicating that possibly the gene flow from tetraploid to hexaploid species was more frequent than that from the diploid Aegilops tauschii [12]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
