Evolutionary conservatism of geographic variation in host preference in Callosobruchus maculatus

Abstract

Abstract. 1. A study of host preference of four pairs of populations of the cowpea weevil Callosobruchus maculates was carried out. The pairs had different geographical origins.2. One population of each pair had been maintained for about 110 generations on cowpea Vigna unguiculata, the other population had been maintained on mung bean V. radiata. Half of the tested females from each population were raised on cowpea and exposed to this host prior to the assay; the other half was raised on mung bean. This design permitted assessment of the relative contributions of geographical origin, recent host use in the laboratory, and individual experience, to variation in host preference.3. Host preference was assayed by letting the females oviposit on an equal‐weight mixture of cowpea and mung seeds; two experiments were performed six generations apart.4. Both experiments revealed a strong effect of geographical origin: populations originating from Nigeria laid a much greater proportion (68–86%) of their eggs on cowpea than those originating from Uganda and Yemen (30–42%); those from Cameroon were intermediate (56–60%). These preferences were not affected consistently by about 110 generations of laboratory evolution on one or the other host, or by experience of individual females.5. These results indicate considerable geographical variation in host preference, and suggest that host preference is behaviourally inflexible and evolutionarily conserved.