Abstract
Chaenomeles (family Rosaceae) is a genus of five diploid species of deciduous spiny shrubs that are native to Central Asia and Japan. It is an important horticultural crop (commonly known as flowering quinces) in Europe and Asia for its high yield in fruits that are rich in juice, aroma, and dietary fiber. Therefore, the development of effective genetic markers of Chaenomeles species is advantageous for crop improvement through breeding and selection. In this study, we successfully assembled and analyzed the chloroplast genome of five Chaenomeles species. The chloroplast genomes of the five Chaenomeles species were very similar with no structural or content rearrangements among them. The chloroplast genomes ranged from 159,436 to 160,040 bp in length and contained a total of 112 unique genes, including 78 protein-coding genes, 30 tRNAs, and 4 rRNAs. Three highly variable regions, including trnR-atpA, trnL-F, and rpl32-ccsA, were identified. Phylogenetic analysis based on the complete chloroplast genome showed that Chaenomeles forms a monophyletic clade and had a close relationship with the genera Docynia and Malus. Analyses for phylogenetic relationships and the development of available genetic markers in future could provide valuable information regarding genetics and breeding mechanisms of the Chaenomeles species.
Highlights
Chaenomeles is a genus of five diploid species of deciduous spiny shrubs that are native to Central Asia and Japan
The complete chloroplast genomes of the five Chaenomeles species ranged from 159,436 bp (C. sinensis) to 160,040 bp (C. cathayensis) in length
We sequenced the total chloroplast genome of five Chaenomeles species by de novo sequencing, and showed that the chloroplast genome structure is well conserved throughout the genus
Summary
Chaenomeles (family Rosaceae) is a genus of five diploid species of deciduous spiny shrubs that are native to Central Asia and Japan It is an important horticultural crop (commonly known as flowering quinces) in Europe and Asia for its high yield in fruits that are rich in juice, aroma, and dietary fiber. C. sinensis (Dum.Cours.) Koehne, referred as Pseudocydonia sinensis (Chinese quince), is a shrub or small tree (5–10 m) that grows in central and southern China. Three of these species (C. cathayensis, C. japonica, and C. speciosa) have been used to create several interspecific hybrids for approximately 400 years, resulting in more than 500 c ultivars[3], with the aim of developing new ornamental cultivars. The development of DNA sequencing technology has resulted in the extensive use of chloroplast genomes for species identification and molecular phylogenetic studies
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